高效液相色谱法（HPLC）结合荧光检测法在临床、制药和环境样品中类固醇定量分析中的应用：综述。

High Performance Liquid Chromatography (HPLC) with Fluorescence Detection for Quantification of Steroids in Clinical, Pharmaceutical, and Environmental Samples: A Review.

机构信息

Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid 22110, Jordan.

School of Biomolecular and Biomedical Science, University College Dublin, Belfield, D04 V1W8 Dublin, Ireland.

出版信息

Molecules. 2022 Mar 10;27(6):1807. doi: 10.3390/molecules27061807.

Steroids are compounds widely available in nature and synthesized for therapeutic and medical purposes. Although several analytical techniques are available for the quantification of steroids, their analysis is challenging due to their low levels and complex matrices of the samples. The efficiency and quick separation of the HPLC combined with the sensitivity, selectivity, simplicity, and cost-efficiency of fluorescence, make HPLC coupled to fluorescence detection (HPLC-FLD) an ideal tool for routine measurement and detection of steroids. In this review, we covered HPLC-FLD methods reported in the literature for the steroids quantification in clinical, pharmaceutical, and environmental applications, focusing on the various approaches of fluorescent derivatization. The aspects related to analytical methodology including sample preparation, derivatization reagents, and chromatographic conditions will be discussed.

甾体化合物广泛存在于自然界中，并被合成用于治疗和医疗目的。尽管有几种分析技术可用于甾体化合物的定量分析，但由于其样品中含量低且基质复杂，分析具有挑战性。HPLC 与荧光检测相结合的高效快速分离与荧光检测的灵敏度、选择性、简单性和成本效益相结合，使 HPLC 与荧光检测（HPLC-FLD）成为常规测量和检测甾体化合物的理想工具。在本综述中，我们涵盖了文献中报道的用于临床、制药和环境应用中甾体化合物定量的 HPLC-FLD 方法，重点介绍了各种荧光衍生化方法。将讨论与分析方法相关的方面，包括样品制备、衍生化试剂和色谱条件。