新西兰肥胖小鼠下丘脑p75肿瘤坏死因子受体表达增加。

Increased hypothalamic expression of the p75 tumor necrosis factor receptor in New Zealand obese mice.

作者信息

Rizk N M, Joost H G, Eckel J

机构信息

Physiology Department, Mansoura Faculty of Medicine, Mansoura University, Egypt.

出版信息

Horm Metab Res. 2001 Sep;33(9):520-4. doi: 10.1055/s-2001-17209.

DOI:10.1055/s-2001-17209

PMID:11561210

Abstract

Previous studies have demonstrated that tumor necrosis factor-alpha (TNF-alpha) production from adipose tissue is elevated in obese animal models and in obese humans. It plays an important role in the induction of insulin resistance in experimental animals. In this study, we examined hypothalamic tissue expression of TNF-alpha and its receptors and TNF-alpha expression of adipose tissue in lean C57BLKSJ+/+ and obese polygenic New Zealand obese (NZO) mice. Obese animals exhibited hyperglycemia, hyperinsulinemia, hypertriglyceridemia, and hypercholesterinemia. Using RT-PCR, we observed increased expression (2.4-fold) of TNF receptor 2 (p75) in the hypothalamus of obese mice. TNF-alpha expression in adipose tissue of obese mice was eight times higher than in controls. TNF-alpha and TNF receptor 1 (p55) expression in hypothalamic tissue was similar in obese and lean animals. These results suggest that the hypothalamic TNF receptor 2 (p75) might play a role in obesity by modulating the actions of TNF-alpha in conditions of leptin resistance.

摘要

以往研究表明，在肥胖动物模型和肥胖人类中，脂肪组织产生的肿瘤坏死因子-α（TNF-α）会升高。它在实验动物胰岛素抵抗的诱导中起重要作用。在本研究中，我们检测了瘦型C57BLKSJ+/+小鼠和肥胖多基因新西兰肥胖（NZO）小鼠下丘脑组织中TNF-α及其受体的表达以及脂肪组织中TNF-α的表达。肥胖动物表现出高血糖、高胰岛素血症、高甘油三酯血症和高胆固醇血症。通过逆转录聚合酶链反应（RT-PCR），我们观察到肥胖小鼠下丘脑肿瘤坏死因子受体2（p75）的表达增加（2.4倍）。肥胖小鼠脂肪组织中TNF-α的表达比对照组高8倍。肥胖和瘦型动物下丘脑组织中TNF-α和肿瘤坏死因子受体1（p55）的表达相似。这些结果表明，下丘脑肿瘤坏死因子受体2（p75）可能在瘦素抵抗情况下通过调节TNF-α的作用而在肥胖中发挥作用。