一种用于固相合成DNA-肽缀合物的新方法。

A novel approach for the solid phase synthesis of DNA-peptide conjugates.

作者信息

Kubo T, Dubey K, Fujii M

机构信息

Department of Chemistry, Kyushu School of Engineering, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):1321-4. doi: 10.1081/NCN-100002546.

DOI:10.1081/NCN-100002546

PMID:11563013

Abstract

The strategy of this study involves automated synthesis of oligonucleotides on a CPG support using standard cyanoethyl phosphoramidite chemistry followed by covalent linkage to peptide fragments bearing a free terminal alpha-amino group and residues with protected side chains. Conjugation was formed through an alkyldiisocyanate linker. Conjugates were isolated by cleavage from the solid support and deprotection in one step.

摘要

本研究的策略包括使用标准的氰乙基亚磷酰胺化学方法在CPG载体上自动合成寡核苷酸，然后与带有游离末端α-氨基和侧链受保护残基的肽片段进行共价连接。通过烷基二异氰酸酯连接子形成缀合物。通过从固相载体上切割并一步脱保护来分离缀合物。