Kubo Takanori, Morikawa Masaaki, Ohba Hideki, Fujii Masayuki
Department of Biological and Environmental Chemistry, Kyushu School of Engineering, Kinki University, 11-6 Kayanomori, Iizuka, Fukuoka 820-8555, Japan.
Org Lett. 2003 Jul 24;5(15):2623-6. doi: 10.1021/ol034721p.
[reaction: see text] DNA-peptide conjugates were synthesized by condensing partially protected peptide fragments and oligonucleotides on a CPG support using diisocyanatoalkane as a linker. After cleavage and deprotection with aqueous ammonia, pure products were obtained by single RPHPLC purification in satisfactory yields and identified by a MALDI-TOF MS spectrometer. This method allows one to prepare DNA-peptide conjugates with any components and sequences of DNA and peptides.
[反应：见正文] 使用二异氰酸酯烷烃作为连接体，通过在CPG载体上缩合部分保护的肽片段和寡核苷酸来合成DNA - 肽缀合物。用氨水进行切割和脱保护后，通过单次反相高效液相色谱（RPHPLC）纯化以令人满意的产率获得纯产物，并通过基质辅助激光解吸电离飞行时间质谱仪（MALDI - TOF MS）进行鉴定。该方法允许制备具有任何DNA和肽的组分及序列的DNA - 肽缀合物。
