NF-κB p50同二聚体对DNA序列的识别：结合序列中的严格识别位点和模糊识别位点

DNA sequence recognition by NF kappa B p50 homodimer: strict and obscure recognition sites in the binding sequence.

作者信息

Kittaka A, Takayama H, Kurihara M, Horii C, Tanaka H, Miyasaka T, Inoue J

机构信息

Faculty of Pharmaceutical Sciences, Teikyo University, Sagamiko, Kanagawa 199-0195, Japan.

出版信息

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):669-72. doi: 10.1081/NCN-100002347.

DOI:10.1081/NCN-100002347

PMID:11563088

Abstract

5-Formyl- and 5-(formylmethyl)-2'-deoxyuridines are introduced into a kappa B site instead of thymidine(s) in order to understand target sequence specificity of NF kappa B. It was found that one thymidine in the kappa B site is particularly important for the sequence specific recognition by NF kappa B.

摘要

为了了解核因子κB（NFκB）的靶序列特异性，将5-甲酰基-2'-脱氧尿苷和5-（甲酰基甲基）-2'-脱氧尿苷引入κB位点以取代胸腺嘧啶。研究发现，κB位点中的一个胸腺嘧啶对于NFκB的序列特异性识别尤为重要。