修饰的含胸腺嘧啶DNA寡核苷酸的合成与生物物理分析
Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.
作者信息
Kawasaki F, Murat P, Li Z, Santner T, Balasubramanian S
机构信息
Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.
出版信息
Chem Commun (Camb). 2017 Jan 24;53(8):1389-1392. doi: 10.1039/c6cc08670e.
Abstract
We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.
摘要
我们报道了5-甲酰基-2'-脱氧尿苷（5fU）亚磷酰胺的合成以及包含所有已知的、天然存在的真核胸腺嘧啶修饰的寡核苷酸的制备。合成寡核苷酸的生物物理表征表明，5fU而非其他胸腺嘧啶衍生物能够改变DNA结构。
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