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修饰的含胸腺嘧啶DNA寡核苷酸的合成与生物物理分析 - Suppr | 超能文献

修饰的含胸腺嘧啶DNA寡核苷酸的合成与生物物理分析

Synthesis and biophysical analysis of modified thymine-containing DNA oligonucleotides.

作者信息

Kawasaki F, Murat P, Li Z, Santner T, Balasubramanian S

机构信息

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK.

出版信息

Chem Commun (Camb). 2017 Jan 24;53(8):1389-1392. doi: 10.1039/c6cc08670e.

DOI:10.1039/c6cc08670e

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5759927/

Abstract

We report the synthesis of a 5-formyl-2'-deoxyuridine (5fU) phosphoramidite and the preparation of oligonucleotides comprising all known, naturally observed eukaryotic thymidine modifications. Biophysical characterization of the synthetic oligonucleotides indicates that 5fU, but not the other T-derivatives, can alter DNA structures.

摘要

我们报道了5-甲酰基-2'-脱氧尿苷（5fU）亚磷酰胺的合成以及包含所有已知的、天然存在的真核胸腺嘧啶修饰的寡核苷酸的制备。合成寡核苷酸的生物物理表征表明，5fU而非其他胸腺嘧啶衍生物能够改变DNA结构。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/4b4fdf636c33/emss-75581-f004.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/4bbbc046ddbb/emss-75581-f001.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/874ae64fdf42/emss-75581-f002.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/f019925a5e95/emss-75581-f003.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/4b4fdf636c33/emss-75581-f004.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/4bbbc046ddbb/emss-75581-f001.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/874ae64fdf42/emss-75581-f002.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/f019925a5e95/emss-75581-f003.jpg

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/229f/5759927/4b4fdf636c33/emss-75581-f004.jpg

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