Wilds C J, Minasov G, Natt F, von Matt P, Altmann K H, Egli M
Department of Biological Sciences, Vanderbilt University, Nashville, Tennessee, USA.
Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):991-4. doi: 10.1081/NCN-100002475.
Chimeric oligodeoxyribonucleotides where the phosphodiester linkage -C3'-O-PO2--O-CH2-C4'- of DNA is substituted by the amide linkage -C3'-CH2-CH*(CH3)-CO-NH-CH2-C4' (*either R or S stereochemistry) have been prepared and their binding to RNA targets have been investigated. Incorporation of a single amide unit increases the Tm by approximately 1.4-1.9 degrees C. Circular dichroic spectra of these modified duplexes are similar to the wildtype DNA/RNA.
已制备出嵌合寡脱氧核糖核苷酸，其中DNA的磷酸二酯键-C3'-O-PO2--O-CH2-C4'被酰胺键-C3'-CH2-CH*(CH3)-CO-NH-CH2-C4'（*为R或S立体化学结构）取代，并对其与RNA靶标的结合进行了研究。单个酰胺单元的掺入使解链温度（Tm）提高了约1.4 - 1.9摄氏度。这些修饰双链体的圆二色光谱与野生型DNA/RNA相似。
