Böcker U, Sirenko O I, Morris J S, Sartor R B, Singer M V, Haskill J S, Watson J M
Center for Gastrointestinal Biology and Diseases, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Immunol Cell Biol. 2001 Oct;79(5):444-53. doi: 10.1046/j.1440-1711.2001.01031.x.
Differences in IL-1beta mRNA expression, stability and translation between non-adherent monocytes and those stimulated by adherence suggest that cytokine regulation is coupled to the function and assembly of cytoskeletal structures. In situ hybridization studies were performed to visualize expression and positioning of IL-1beta mRNA in adherently cultivated monocytes. IL-1beta mRNA expression was heterogeneous with high transcript levels found in spread or polarized cells. Transcripts were compartmentalized to the perinuclear region in spread cells, and partially redistributed with polarization. In contrast to mRNA distribution in other motile cell populations, IL-1beta mRNA did not localize to the distal or proximal actin cytoskeleton. Perinuclear confinement of transcripts required intact actin microfilaments. Treatment with cytoskeleton disruption and detergent extraction suggested that most non-translated IL-1beta mRNA was associated with intermediate filaments. In monocytes stimulated by LPS, IL-1beta, but not IL-1Ra transcripts were redistributed and partially associated, yet not bound to actin microfilaments. The present study demonstrates that IL-1beta mRNA expression and localization in adherent monocytes is interrelated with the cytoskeletal rearrangement upon adherence, spreading and polarization.
非贴壁单核细胞与贴壁刺激的单核细胞之间白细胞介素-1β（IL-1β）信使核糖核酸（mRNA）表达、稳定性及翻译的差异表明，细胞因子调节与细胞骨架结构的功能及组装相关联。进行原位杂交研究以观察IL-1β mRNA在贴壁培养单核细胞中的表达及定位情况。IL-1β mRNA表达具有异质性，在铺展或极化细胞中发现有高转录水平。转录本在铺展细胞中被分隔在核周区域，并随着极化而部分重新分布。与其他运动细胞群体中的mRNA分布不同，IL-1β mRNA并不定位于远端或近端肌动蛋白细胞骨架。转录本的核周定位需要完整的肌动蛋白微丝。细胞骨架破坏和去污剂提取处理表明，大多数未翻译的IL-1β mRNA与中间丝相关联。在脂多糖（LPS）刺激的单核细胞中，IL-1β而非白细胞介素-1受体拮抗剂（IL-1Ra）转录本重新分布并部分相关联，但未与肌动蛋白微丝结合。本研究表明，贴壁单核细胞中IL-1β mRNA的表达及定位与贴壁、铺展和极化时的细胞骨架重排相互关联。
