Bohnhorst J Ø, Bjørgan M B, Thoen J E, Natvig J B, Thompson K M
Institute of Immunology and Centre for Rheumatic Disease, Rikshospitalet, University Hospital, University of Oslo, N-0027 Oslo, Norway.
J Immunol. 2001 Oct 1;167(7):3610-8. doi: 10.4049/jimmunol.167.7.3610.
Analyses of B cells in the bone marrow and secondary lymphoid tissues have revealed a broad range of cell surface markers defining B cell subpopulations, but only a few of these have been used to analyze B cell subpopulations in peripheral blood (PB). We report here the delineation of circulating PB B cell subpopulations by staining for CD19, CD38, and IgD in combination with CD10, CD44, CD77, CD95, CD23, IgM, and the B cell memory marker CD27. The utility of this approach is shown by the demonstration of disturbances of circulating B cell subpopulations in patients with autoimmune disease. Five mature B cell (Bm) subpopulations were identified in normal PB that were comparable with the tonsillar Bm1, Bm2, early Bm5, Bm5 subpopulations and, surprisingly, to the germinal center (GC) founder cell subpopulation (Bm2' and Bm3delta-4delta), suggesting that some GC founder cells are circulating. No PB B cells resembled the Bm3 and Bm4 GC cells. Remarkably, some cells with the CD38-IgD+ phenotype, previously known as naive Bm1 cells, expressed CD27. The CD38-IgD+ subpopulation therefore includes both naive Bm1 cells and IgD+ memory B cells. This new classification of B cell developmental stages reveals disturbances in the proportions of B cell subpopulations in primary Sjögren's syndrome (pSS) patients compared with healthy donors and rheumatoid arthritis patients. Patients with pSS contained a significantly higher percentage of B cells in two activated stages, which might reflect a disturbance in B cell trafficking and/or alteration in B cell differentiation. These findings could be of diagnostic significance for pSS.
对骨髓和二级淋巴组织中的B细胞分析显示，有多种细胞表面标志物可定义B细胞亚群，但其中只有少数用于分析外周血（PB）中的B细胞亚群。我们在此报告通过联合检测CD19、CD38、IgD以及CD10、CD44、CD77、CD95、CD23、IgM和B细胞记忆标志物CD27来描绘循环外周血B细胞亚群。自身免疫性疾病患者循环B细胞亚群紊乱的实例表明了该方法的实用性。在正常外周血中鉴定出五个成熟B细胞（Bm）亚群，它们与扁桃体Bm1、Bm2、早期Bm5、Bm5亚群相当，令人惊讶的是，还与生发中心（GC）起始细胞亚群（Bm2'和Bm3δ-4δ）相当，这表明一些GC起始细胞在循环。外周血中没有B细胞类似于Bm3和Bm4 GC细胞。值得注意的是，一些具有CD38-IgD+表型的细胞，以前被称为幼稚Bm1细胞，表达CD27。因此，CD38-IgD+亚群既包括幼稚Bm1细胞，也包括IgD+记忆B细胞。这种B细胞发育阶段的新分类揭示了原发性干燥综合征（pSS）患者与健康供体和类风湿关节炎患者相比，B细胞亚群比例存在紊乱。pSS患者在两个活化阶段的B细胞百分比显著更高，这可能反映了B细胞转运的紊乱和/或B细胞分化的改变。这些发现可能对pSS具有诊断意义。
