Atkins K B, Johns D, Watts S, Clinton Webb R, Brosius F C
Department of Internal Medicine, University of Michigan Medical School, East Lansing, Michigan 48109-0676, USA.
J Hypertens. 2001 Sep;19(9):1581-7. doi: 10.1097/00004872-200109000-00009.
Because glucose uptake and metabolism can affect vascular smooth muscle cell function, we proposed that animals with hypertension might develop alterations in glucose transporter expression in vascular smooth muscle cells that were responsible for some of the vascular abnormalities characteristic of hypertension.
Male Sprague-Dawley rats (250-300 g) were left uni-nephrectomized and either implanted or not with deoxycorticosterone acetate (DOCA, 200 mg/kg) impregnated silastic. All animals were fed normal rat chow. The DOCA-implanted rats were given water supplemented to 1% NaCl and 0.2% KCl for 7, 14 or 28 days.
The insulin-response glucose transporter (GLUT4) polypeptide levels were depressed several-fold in aortae and carotid arteries from DOCA-salt hypertensive rats compared with sham rats. Uptake of the glucose analog, 2-deoxyglucose (2-DOG), was also reduced 53% in hypertensive compared with sham aortae. There were no changes in GLUT4 expression in other tissues in the DOCA-salt animals, nor were there significant changes in aortae from spontaneously hypertensive rat/stroke prone animals. As previously demonstrated, carotid arteries from DOCA-salt animals exhibited a significant increased contractile sensitivity to ergonovine. Inhibition of glucose metabolism with 2-DOG in sham arteries caused a marked enhancement of contractile responsiveness to ergonovine, whereas 2-DOG had no effect on the already enhanced contractility of DOCA-salt arteries, suggesting that reduction in glucose uptake and metabolism substantially increases the contractile response of DOCA-salt arteries.
Alterations in glucose uptake and metabolism in vascular smooth muscle cells may participate in the contractile abnormalities characteristic of certain forms of hypertension.
由于葡萄糖摄取和代谢会影响血管平滑肌细胞功能，我们推测高血压动物的血管平滑肌细胞中葡萄糖转运蛋白表达可能发生改变，这与高血压特有的一些血管异常有关。
雄性Sprague-Dawley大鼠（250 - 300克）单侧肾切除，部分植入或未植入醋酸脱氧皮质酮（DOCA，200毫克/千克）浸渍的硅橡胶。所有动物均喂食正常大鼠饲料。给植入DOCA的大鼠饮用添加了1%氯化钠和0.2%氯化钾的水，持续7、14或28天。
与假手术大鼠相比，DOCA-盐高血压大鼠的主动脉和颈动脉中胰岛素反应性葡萄糖转运蛋白（GLUT4）多肽水平降低了数倍。与假手术主动脉相比，高血压大鼠对葡萄糖类似物2-脱氧葡萄糖（2-DOG）的摄取也减少了53%。DOCA-盐动物其他组织中的GLUT4表达没有变化，自发性高血压大鼠/易中风动物的主动脉也没有显著变化。如先前所示，DOCA-盐动物的颈动脉对麦角新碱的收缩敏感性显著增加。用2-DOG抑制假手术动脉中的葡萄糖代谢会导致对麦角新碱的收缩反应性显著增强，而2-DOG对DOCA-盐动脉已经增强的收缩性没有影响，这表明葡萄糖摄取和代谢的减少会大幅增加DOCA-盐动脉的收缩反应。
血管平滑肌细胞中葡萄糖摄取和代谢的改变可能参与了某些形式高血压特有的收缩异常。
