Palotás A, Kálmán J, Laskay G, Juhász A, Janka Z, Penke B
Department of Medical Chemistry, University of Szeged, Hungary.
Neurochem Res. 2001 Jul;26(7):817-20. doi: 10.1023/a:1011664102849.
The accumulation of the beta-amyloid peptide (betaAP) in the brain, produced from the ubiquitously expressed amyloid precursor protein (APP) is a defining feature of Alzheimer's disease (AD). Consistent with studies demonstrating the importance of skin biopsy in the diagnosis of neurodegenerative disorders, we investigated whether differences in intracellular free calcium levels ([Ca2+]i) of cultured cutaneous fibroblasts derived from sporadic AD patients and from age-matched control individuals might be present. [Ca2+]i was measured in Fura-2AM-loaded human fibroblasts by dual wavelength spectrofluorimetry. AD cells exhibited lower [Ca2+]i as compared to the control cultures. Exposure of fibroblasts to betaAP resulted in increased [Ca2+]i of the control cells, but not of AD fibroblasts. Our test could prove useful in supporting the diagnosis of (sporadic) AD in patients suspected of suffering from the disease.
由普遍表达的淀粉样前体蛋白（APP）产生的β-淀粉样肽（βAP）在大脑中的积累是阿尔茨海默病（AD）的一个决定性特征。与证明皮肤活检在神经退行性疾病诊断中的重要性的研究一致，我们调查了散发性AD患者和年龄匹配的对照个体来源的培养皮肤成纤维细胞的细胞内游离钙水平（[Ca2+]i）是否存在差异。通过双波长荧光分光光度法在负载Fura-2AM的人成纤维细胞中测量[Ca2+]i。与对照培养物相比，AD细胞表现出较低的[Ca2+]i。将成纤维细胞暴露于βAP导致对照细胞的[Ca2+]i增加，但AD成纤维细胞没有。我们的测试可能有助于支持对疑似患有该疾病的患者进行（散发性）AD的诊断。
