Wait R, Gianazza E, Eberini I, Sironi L, Dunn M J, Gemeiner M, Miller I
Kennedy Institute of Rheumatology Division, Imperial College School of Medicine, Hammersmith, London, UK.
Electrophoresis. 2001 Aug;22(14):3043-52. doi: 10.1002/1522-2683(200108)22:14<3043::AID-ELPS3043>3.0.CO;2-M.
We have investigated the biological fluids--serum, cerebrospinal fluid, and urine--of three strains of rats; the present data extend our database (also available on-line) and may be of interest for pharmacological and toxicological investigation. Specifically, we have defined reference maps of the major protein components in cerebrospinal fluid and urine. Compartment-specific isoforms were recognized for transferrin and transthyretin. Mass spectrometric data established the cleavage site of the signal peptide and identified the N-terminal blocking group of prostaglandin D synthase from rat cerebrospinal fluid. A previously undescribed member of the family of low molecular mass rat urinary proteins was characterized as containing a sequence similar, but not identical, to the N-terminal region of rat urinary protein-2 (RUP-2), and divergent from RUP-1.
我们研究了三种品系大鼠的生物体液——血清、脑脊液和尿液；目前的数据扩展了我们的数据库（也可在线获取），可能对药理学和毒理学研究具有重要意义。具体而言，我们已经确定了脑脊液和尿液中主要蛋白质成分的参考图谱。识别出了转铁蛋白和甲状腺素运载蛋白的特定区室同工型。质谱数据确定了信号肽的切割位点，并鉴定了大鼠脑脊液中前列腺素D合酶的N端封闭基团。一种此前未被描述的低分子量大鼠尿液蛋白家族成员，其特征在于含有与大鼠尿液蛋白-2（RUP-2）的N端区域相似但不完全相同的序列，且与RUP-1不同。
