Reem G H
Ciba Found Symp. 1978(68):207-11. doi: 10.1002/9780470720516.ch13.
An antibody prepared against human placental purine nucleoside phosphorylase (PNP) has been used to characterize the subunit structure of the normal enzyme by two-dimensional gel electrophoresis. The antibody against the placental enzyme reacts with PNP present in human lymphocytes, fibroblasts, erythrocytes and placenta. The enzyme can be detected in intact cells by immunofluorescence with the same antibody preparation. The techniques allow the identification of cross-reacting material in a small number of lymphocytes and could therefore be used to detect abnormal enzyme protein in immunodeficiency.
一种针对人胎盘嘌呤核苷磷酸化酶（PNP）制备的抗体，已被用于通过二维凝胶电泳来表征正常酶的亚基结构。针对胎盘酶的抗体可与人淋巴细胞、成纤维细胞、红细胞和胎盘中存在的PNP发生反应。使用相同的抗体制备物，通过免疫荧光可在完整细胞中检测到该酶。这些技术能够鉴定少量淋巴细胞中的交叉反应物质，因此可用于检测免疫缺陷中异常的酶蛋白。
