Boland S, Baeza-Squiban A, Bonvallot V, Houcine O, Pain C, Meyer M, Marano F
Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Université Paris VII Denis Diderot, Tour 53/54 E3, case 70-73, 2 place Jussieu, 75251 Paris cédex 05, France.
Toxicol In Vitro. 2001 Aug-Oct;15(4-5):379-85. doi: 10.1016/s0887-2333(01)00040-6.
Standard reference diesel exhaust particles (DEP) SRM 1650 are often used to evaluate the toxicity of DEP. However, these particles did not necessarily reflect the effects of DEP representative of present diesel automobiles. This study was designed to compare the effects of SRM 1650 to DEP from representative cars (RC-DEP) on airway epithelial cells. Therefore we established a method to recover RC-DEP impacted on filters after emission from diesel automobiles on test beds. Electron microscopy and flow cytometry showed that these two types of particles were phagocytosed to the same extent by epithelial cells. This phagocytosis is not dependent on the adsorbed organic compounds in contrast to the cytotoxic effect evaluated by measurements of LDH release. This is emphasized by the fact that RC-DEP equipped with an oxidation catalyst are less cytotoxic than particles from a non-equipped vehicle or SRM 1650. This type of catalyst also reduces significantly the release of GM-CSF by bronchial epithelial cells. We have shown in the present paper that SRM 1650 may be used as a surrogate of DEP. However, exhaust gas post-treatment devices of current diesel automobiles reduce the cytotoxicity as well as the inflammatory response of these particles.
标准参考柴油尾气颗粒（DEP）SRM 1650常被用于评估DEP的毒性。然而，这些颗粒不一定能反映当前柴油汽车典型DEP的影响。本研究旨在比较SRM 1650与典型汽车的DEP（RC-DEP）对气道上皮细胞的影响。因此，我们建立了一种方法，用于回收在试验台上柴油汽车排放后附着在滤器上的RC-DEP。电子显微镜和流式细胞术显示，这两种类型的颗粒被上皮细胞吞噬的程度相同。与通过测量乳酸脱氢酶（LDH）释放评估的细胞毒性作用不同，这种吞噬作用不依赖于吸附的有机化合物。配备氧化催化剂的RC-DEP的细胞毒性低于未配备车辆的颗粒或SRM 1650，这一事实强调了这一点。这种类型的催化剂还能显著减少支气管上皮细胞释放粒细胞-巨噬细胞集落刺激因子（GM-CSF）。我们在本文中表明，SRM 1650可作为DEP的替代物。然而，当前柴油汽车的废气后处理装置降低了这些颗粒的细胞毒性以及炎症反应。
