Caggiari L, Zanussi S, D'Andrea M, Bortolin M T, Crepaldi C, Caffau C, Paoli P D
Microbiology, Immunology and Virology, Centro di Riferimento Oncologico, Aviano, Italy.
Eur Cytokine Netw. 2001 Jul-Sep;12(3):430-6.
Interleukin-2 has been widely used in HIV-1+ subjects as an immunoactivating agent. In this study, we investigated cytokine production, Ki67 antigen expression and the modulation of the surface phenotype of the CD4/CD25+ subset as compared to the reciprocal CD4/CD25- subset in IL-2-treated HIV+ patients. Our findings suggest that CD4 T cells are heterogeneous in responding to IL-2, because CD4/CD25+ cells sharply increased their "memory" phenotype, their Ki67 antigen expression and were the main in vivo targets for IL-2-dependent proliferation during therapy, while the percentages of IFN-gamma+ (terminally differentiated) cells remained unchanged at the end of therapy. Conversely, the CD4+/CD25- subpopulation showed an expansion of differentiated cells and a slight increase in the proliferation rate. The use of anti-retroviral therapy alone (HAART) reduced the proliferation and increased the differentiation of both CD4 subsets. Our data suggest that IL-2 has a moderate capacity to activate resting T cells in vivo and is probably unable to boost HIV-1 from latency to the replicative state.
白细胞介素-2作为一种免疫激活剂已被广泛应用于HIV-1阳性个体。在本研究中，我们调查了在接受白细胞介素-2治疗的HIV阳性患者中，与相应的CD4/CD25-亚群相比，细胞因子的产生、Ki67抗原的表达以及CD4/CD25+亚群表面表型的调节情况。我们的研究结果表明，CD4 T细胞对白细胞介素-2的反应具有异质性，因为CD4/CD25+细胞显著增加了其“记忆”表型、Ki67抗原的表达，并且是治疗期间白细胞介素-2依赖性增殖的主要体内靶点，而在治疗结束时，IFN-γ+（终末分化）细胞的百分比保持不变。相反，CD4+/CD25-亚群显示分化细胞增多，增殖率略有增加。单独使用抗逆转录病毒疗法（HAART）可降低两个CD4亚群的增殖并增加其分化。我们的数据表明，白细胞介素-2在体内激活静息T细胞的能力适中，可能无法将HIV-1从潜伏状态激活至复制状态。
