Ji Y, Zhang B, Van S F, Warren P, Woodnutt G, Burnham M K, Rosenberg M
Department of Microbiology, Genetics Research, GlaxoSmithKline Pharmaceuticals Research and Development, Collegeville, PA 19426, USA.
Science. 2001 Sep 21;293(5538):2266-9. doi: 10.1126/science.1063566.
Comprehensive genomic analysis of the important human pathogen Staphylococcus aureus was achieved by a strategy involving antisense technology in a regulatable gene expression system. In addition to known essential genes, many genes of unknown or poorly defined biological function were identified. This methodology allowed gene function to be characterized in a comprehensive, defined set of conditionally growth-defective/lethal isogenic strains. Quantitative titration of the conditional growth effect was performed either in bacterial culture or in an animal model of infection. This genomic strategy offers an approach to the identification of staphylococcal gene products that could serve as targets for antibiotic discovery.
通过在可调控基因表达系统中运用反义技术的策略，对重要的人类病原体金黄色葡萄球菌进行了全面的基因组分析。除了已知的必需基因外，还鉴定出许多生物学功能未知或定义不明确的基因。这种方法能够在一组全面的、明确的条件性生长缺陷/致死性同基因菌株中表征基因功能。在细菌培养物或感染动物模型中对条件性生长效应进行了定量滴定。这种基因组策略为鉴定可作为抗生素发现靶点的葡萄球菌基因产物提供了一种方法。
