Miyata T, Kawaguchi A, Okano H, Ogawa M
Laboratory for Cell Culture Development, Brain Science Institute, RIKEN, 351-0198, Saitama, Japan.
Neuron. 2001 Sep 13;31(5):727-41. doi: 10.1016/s0896-6273(01)00420-2.
Recent studies demonstrated the neuronogenic role of radial glial cells (RGCs) in the rodent. To reveal the fate of radial glial processes, we intensively monitored divisions of RGCs in DiI-labeled slices from the embryonic day 14 mouse cortex. During RGC division, each pia-connected fiber becomes thin but is neither lost nor divided; it is inherited asymmetrically by one daughter cell. In divisions that produce a neuron and a progenitor, the neuron inherits the pial fiber, also grows a thick ventricular process for several hours, and is therefore indistinguishable from the progenitor RGC. The ventricular process in the radial glial-like neuron ("radial neuron") then collapses, leading to ascent of the neuron by using the "recycled" radial fiber.
最近的研究证实了啮齿动物中放射状胶质细胞（RGCs）的神经发生作用。为了揭示放射状胶质细胞突起的命运，我们密切监测了来自胚胎第14天小鼠皮质的DiI标记切片中RGCs的分裂情况。在RGC分裂过程中，每条与软脑膜相连的纤维会变细，但既不会丢失也不会分裂；它由一个子细胞不对称继承。在产生一个神经元和一个祖细胞的分裂中，神经元继承软脑膜纤维，也会在几个小时内长出一条粗大的室管膜突起，因此与祖细胞RGC难以区分。然后，放射状胶质样神经元（“放射状神经元”）中的室管膜突起会塌陷，利用“回收利用”的放射状纤维使神经元上升。
