Shires J, Theodoridis E, Hayday A C
Peter Gorer Department of Immunobiology, Guy's, King's, and St. Thomas' Medical School, King's College, University of London, London Bridge, London SE1 9RT, United Kingdom.
Immunity. 2001 Sep;15(3):419-34. doi: 10.1016/s1074-7613(01)00192-3.
Intraepithelial lymphocytes (IELs) are abundant, evolutionarily conserved T cells, commonly enriched in T cell receptor (TCR) gammadelta expression. However, their primary functional potential and constitutive activation state are incompletely understood. To address this, serial analysis of gene expression (SAGE) was applied to murine TCRgammadelta+ and TCRalphabeta+ intestinal IELs directly ex vivo, identifying 15,574 unique transcripts that collectively portray an "activated yet resting," Th1-skewed, cytolytic, and immunoregulatory phenotype applicable to multiple subsets of gut IELs. Expression of granzymes, Fas ligand, RANTES, prothymosin beta4, junB, RGS1, Btg1, and related molecules is high, whereas expression of conventional cytokines and high-affinity cytokine receptors is low. Differentially expressed genes readily identify heterogeneity among TCRalphabeta+ IELs, whereas differences between resident TCRgammadelta+ IELs and TCRalphabeta+ IELs are less obvious.
上皮内淋巴细胞（IELs）是丰富的、进化上保守的T细胞，通常富含T细胞受体（TCR）γδ表达。然而，它们的主要功能潜力和组成性激活状态尚未完全了解。为了解决这个问题，基因表达序列分析（SAGE）被直接应用于体外的小鼠TCRγδ+和TCRαβ+肠道IELs，鉴定出15,574个独特的转录本，这些转录本共同描绘了一种“激活但静止”、Th1偏向、细胞溶解和免疫调节的表型，适用于肠道IELs的多个亚群。颗粒酶、Fas配体、RANTES、前胸腺素β4、junB、RGS1、Btg1及相关分子的表达较高，而传统细胞因子和高亲和力细胞因子受体的表达较低。差异表达基因很容易识别TCRαβ+ IELs之间的异质性，而驻留TCRγδ+ IELs和TCRαβ+ IELs之间的差异则不太明显。
