左旋肉碱对三碘甲状腺原氨酸和脂肪酸诱导的膜通透性转变的抑制作用。

Suppression of T(3)- and fatty acid-induced membrane permeability transition by L-carnitine.

作者信息

Kashiwagi A, Kanno T, Arita K, Ishisaka R, Utsumi T, Utsumi K

机构信息

Laboratory for Amphibian Biology, Graduate School of Science, Hiroshima University, 739-8526, Higashihiroshima, Japan.

出版信息

Comp Biochem Physiol B Biochem Mol Biol. 2001 Oct;130(3):411-8. doi: 10.1016/s1096-4959(01)00458-4.

DOI:10.1016/s1096-4959(01)00458-4

PMID:11567904

Abstract

Cytochrome c (Cyt. c) is known to be released from the mitochondria into the cytosol by means of the membrane permeability transition (MPT) mechanism, thereby activating caspase cascade activity, and inducing cell apoptosis. Recently we reported that L-carnitine suppressed palmitoyl-CoA-induced MPT as well as apoptosis in some cell types (Biochem. Pharmacol, in press). In the present study T(3) was found to induce MPT and Cyt. c release, while cyclosporin A (CsA), bovine serum albumin (BSA) and L-carnitine were found to inhibit this action in a concentration-dependent manner. Similarly, long chain fatty acid (LCFA) also induced MPT and Cyt. c release, which was then inhibited by CsA, BSA and L-carnitine. From these results the authors postulate that T(3)-induced MPT is in part regulated by fatty acid metabolism through a dynamic balance between LCFAs and L-carnitine.

摘要

细胞色素c（Cyt. c）已知通过膜通透性转换（MPT）机制从线粒体释放到细胞质中，从而激活半胱天冬酶级联活性，并诱导细胞凋亡。最近我们报道，L-肉碱在某些细胞类型中抑制棕榈酰辅酶A诱导的MPT以及凋亡（《生物化学与药理学》，即将发表）。在本研究中，发现T3诱导MPT和Cyt. c释放，而环孢菌素A（CsA）、牛血清白蛋白（BSA）和L-肉碱以浓度依赖性方式抑制这种作用。同样，长链脂肪酸（LCFA）也诱导MPT和Cyt. c释放，随后被CsA、BSA和L-肉碱抑制。基于这些结果，作者推测T3诱导的MPT部分受脂肪酸代谢调节，通过LCFAs和L-肉碱之间的动态平衡实现。