将Bio-Rad %CDT TIA和CDTect作为重度饮酒实验室标志物的比较及其与γ-谷氨酰转移酶的关系。

Comparison of Bio-Rad %CDT TIA and CDTect as laboratory markers of heavy alcohol use and their relationships with gamma-glutamyltransferase.

作者信息

Anton R F, Dominick C, Bigelow M, Westby C

机构信息

Clinical Neurobiology Laboratories and Alcohol Research Center, Department of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC 29425, USA.

出版信息

Clin Chem. 2001 Oct;47(10):1769-75.

PMID:11568085

Abstract

BACKGROUND

Carbohydrate-deficient transferrin (CDT) is used as a serum marker for heavy drinking. We compared a new Bio-Rad %CDT TIA assay with the CDTect assay; we also compared both to gamma-glutamyltransferase (GGT) as markers of heavy drinking.

METHODS

Serum samples of well-defined alcoholics (n = 404) and matched (age, race, and gender) social drinkers (204) from 10 clinical centers were assayed with both CDT assays. Both assays use microcolumn separation after iron saturation, followed by enzyme immunoassay (CDTect) or turbidimetric immunoassay (Bio-Rad %CDT). In the latter, CDT is expressed as a percentage of total transferrin.

RESULTS

The slope and intercept [95% confidence intervals (CIs)] for linear regression of results obtained by the %CDT-TIA (as percentage) and CDTect (units/L) assays were 0.091 (0.088-0.097) and 0.70% (0.54-0.86%), respectively (S(y/x) =1.30%; r = 0.848). The areas under the ROC curves (95% CIs) for CDTect and Bio-Rad %CDT TIA were 0.89 (0.86-0.92) and 0.88 (0.85-0.91), respectively, for men (P, not significant) and 0.76 (0.72-0.80) and 0.72 (0.68-0.76) for women (P, not significant). When CDT (CDTect or Bio-Rad %CDT) was combined with GGT (either one positive), the clinical sensitivity in men was 90% for both assays, and specificities were 81% and 84%, respectively; sensitivities in women were 75% and 76%, respectively, and specificities were 87% and 91%.

CONCLUSION

The new Bio-Rad %CDT TIA assay compares favorably to the widely studied CDTect assay in the detection of alcohol-use disorders.

摘要

背景

缺糖转铁蛋白（CDT）用作重度饮酒的血清标志物。我们将Bio-Rad公司新的%CDT TIA检测法与CDTect检测法进行了比较；我们还将这两种检测法与γ-谷氨酰转移酶（GGT）作为重度饮酒的标志物进行了比较。

方法

来自10个临床中心的明确诊断的酗酒者（n = 404）和匹配的（年龄、种族和性别）社交饮酒者（204）的血清样本用两种CDT检测法进行检测。两种检测法均在铁饱和后使用微柱分离，随后进行酶免疫测定（CDTect）或比浊免疫测定（Bio-Rad %CDT）。在后者中，CDT表示为总转铁蛋白的百分比。

结果

%CDT-TIA（以百分比计）和CDTect（单位/L）检测法所得结果的线性回归斜率和截距[95%置信区间（CI）]分别为0.091（0.088 - 0.097）和0.70%（0.54 - 0.86%）（S(y/x) = 1.30%；r = 0.848）。男性中，CDTect和Bio-Rad %CDT TIA的ROC曲线下面积（95% CI）分别为0.89（0.8 June 6 - 0.92）和0.88（0.85 - 0.91）（P，无显著性差异），女性中分别为0.76（0.72 - 0.80）和0.72（0.68 - 0.76）（P，无显著性差异）。当CDT（CDTect或Bio-Rad %CDT）与GGT（任一阳性）联合使用时，两种检测法在男性中的临床敏感性均为90%，特异性分别为81%和84%；女性中的敏感性分别为75%和76%，特异性分别为87%和91%。