在利用靶向肿瘤发生技术构建的新型肾上腺细胞系中，硒对类固醇生成的调节作用。
Modulation of steroidogenesis by selenium in a novel adrenal cell line developed using targeted tumorigenesis.
作者信息
Chanoine J P, Compagnone N A, Wong A C, Mellon S H
机构信息
Department of Pediatrics, Endocrine and Diabetes Unit, University of British Columbia, Vancouver V5Z 4H4, BC, Canada.
出版信息
Biofactors. 2001;14(1-4):229-38. doi: 10.1002/biof.5520140129.
UNLABELLED
Glutathione peroxidase (GPx-1) is a selenoenzyme that metabolizes H(2)O(2), a source of potentially toxic free radicals. Steroidogenesis is markedly inhibited by H(2)O(2) in vitro.
OBJECTIVE
to study the effects of selenium deficiency on GPx activity and adrenal steroidogenesis in a novel adrenal cell line developed using targeted tumorigenesis.
METHODS
AN4Rppc7 cells were grown for 7 days in serum-free medium. 8-Br-cAMP-stimulated concentrations of steroid hormones were measured by RIA. StAR (Steroid Acute Reactive Protein) mRNA was measured by Northern blot.
RESULTS
selenium deficiency caused a 99% There was a 51%, progesterone, corticosterone and aldosterone production, respectively (p<0.05 by ANOVA). StAR mRNA was not affected by selenium.
CONCLUSIONS
selenium deficiency causes a marked decrease in GPx activity. Decreased steroid hormone production occurs for selenium concentrations equal or lower than 5 nM. The absence of changes in StAR mRNA content suggests that selenium deficiency does not affect cholesterol access to the mitochondria.
未标记
谷胱甘肽过氧化物酶（GPx-1）是一种硒酶，可代谢H₂O₂，H₂O₂是潜在有毒自由基的来源。在体外，H₂O₂可显著抑制类固醇生成。
目的
利用靶向肿瘤发生技术建立一种新型肾上腺细胞系，研究硒缺乏对GPx活性和肾上腺类固醇生成的影响。
方法
将AN4Rppc7细胞在无血清培养基中培养7天。用放射免疫分析法测定8-溴环磷腺苷刺激后的类固醇激素浓度。用Northern印迹法测定类固醇急性反应蛋白（StAR）mRNA。
结果
硒缺乏导致GPx活性降低99%。孕酮、皮质酮和醛固酮的生成分别减少51%（方差分析，p<0.05）。StAR mRNA不受硒的影响。
结论
硒缺乏导致GPx活性显著降低。当硒浓度等于或低于5 nM时，类固醇激素生成减少。StAR mRNA含量无变化表明硒缺乏不影响胆固醇进入线粒体。
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