Suzue G, Marcel Y L
Can J Biochem. 1975 Jul;53(7):804-9. doi: 10.1139/o75-108.
Gel filtration on Sephadex G-75 of crude rat liver supernatant preincubated with [1-14C]oleic acid yields three peaks of radioactivity which are attributed to the presence in these fractions of fatty acid binding proteins. We have confirmed these observations with binding assays by phase partition, polyacrylamide gel electrophoresis, and thin layer electrofocusing. Peak I (mol. wt. 60,000 pI 5.01 was shown to be albumin, which mainly arises from a contamination of the liver preparation by blood. Peak II (mol. wt. 10,000, pI 5.9) is a fatty acid binding protein. Finally peak III (mol. wt. 1500, pI 5.7) is a fatty acid binding component, the chemical nature of which was not elucidated. These fatty acid binding fractions have no effect on the reaction of acyl-CoA synthetase whereas the crude liver supernatant does stimulate the activation of fatty acid as shown earlier. In consequence, the physiological role of these fatty acid binding fractions is not yet elucidated.
用葡聚糖凝胶G - 75对预先与[1 - ¹⁴C]油酸温育的大鼠肝脏粗提上清液进行凝胶过滤，得到三个放射性峰，这归因于这些组分中存在脂肪酸结合蛋白。我们通过相分配结合测定、聚丙烯酰胺凝胶电泳和薄层层析聚焦法证实了这些观察结果。峰I（分子量60,000，等电点5.01）被证明是白蛋白，它主要源于肝脏制剂被血液污染。峰II（分子量10,000，等电点5.9）是一种脂肪酸结合蛋白。最后，峰III（分子量1500，等电点5.7）是一种脂肪酸结合成分，其化学性质尚未阐明。这些脂肪酸结合组分对酰基辅酶A合成酶的反应没有影响，而如前所示，肝脏粗提上清液确实能刺激脂肪酸的活化。因此，这些脂肪酸结合组分的生理作用尚未阐明。
