Landman W J, van Eck J H
Animal Health Service, Poultry Health Center, Deventer, The Netherlands.
Avian Dis. 2001 Jul-Sep;45(3):684-7.
In order to study the aerosol transmission of arthropathic and amyloidogenic Enterococcus faecalis strains, preliminary aerosol experiments were performed. The experiments were carried out in empty isolators to assess the yield and viability of E. faecalis and Newcastle disease vaccine virus (NDVV) aerosol particles with time. NDVV was aerosolized because this virus would be used in combination with E. faecalis in a subsequent study. Concentrations of about 10(5) colony-forming units (CFU) of E. faecalis/ml of air were still found 30 min after the aerosol application. At 45 min, however, E. faecalis concentrations dropped below the detection level. The average E. faecalis concentration during the aerosol experiment was estimated at 10(5) CFU/liter. The NDVV aerosol generated an average of 10(4)-10(5) 50% embryo infective dose per liter of air. In these experiments, E. faecalis and NDVV aerosols were successfully generated despite considerable initial particle loss. The bacteria and virus uptakes per chick are discussed in case day-old chicks would be exposed to these aerosols.
为了研究关节炎性和淀粉样变粪肠球菌菌株的气溶胶传播，进行了初步的气溶胶实验。实验在空的隔离器中进行，以评估粪肠球菌和新城疫疫苗病毒（NDVV）气溶胶颗粒的产量和活力随时间的变化。选择NDVV进行气溶胶化是因为该病毒将在后续研究中与粪肠球菌联合使用。气溶胶施加30分钟后，仍可在空气中检测到浓度约为每毫升10⁵菌落形成单位（CFU）的粪肠球菌。然而，在45分钟时，粪肠球菌浓度降至检测水平以下。气溶胶实验期间粪肠球菌的平均浓度估计为每升10⁵CFU。NDVV气溶胶每升空气平均产生10⁴ - 10⁵个50%胚感染剂量。在这些实验中，尽管初始颗粒有相当大的损失，但仍成功产生了粪肠球菌和NDVV气溶胶。还讨论了如果一日龄雏鸡暴露于这些气溶胶时每只雏鸡对细菌和病毒的摄取情况。
