在水/四氯甲烷界面利用金霉素-铕-三辛基氧化膦复合阳离子通过全内反射共振光散射法测定体液中的金霉素。

Total internal reflected resonance light scattering determination of chlortetracycline in body fluid with the complex cation of chlortetracycline-europium-trioctyl phosphine oxide at the water/tetrachloromethane interface.

作者信息

Feng P, Shu W Q, Huang C Z, Li Y F

机构信息

Institute of Environmental Chemistry, Southwest Normal University, Chongqing, People's Republic of China.

出版信息

Anal Chem. 2001 Sep 1;73(17):4307-12. doi: 10.1021/ac001514j.

DOI:10.1021/ac001514j

PMID:11569824

Abstract

A highly selective method of chlortetracycline (CTC) is proposed on the basis of the measurements of total internal reflected resonance light scattering (TMR-RLS) at water/tetrachloromethane (H20/CCl4) interfaces. In the pH range of 7.54-8.14, the interaction of the binary complex of Eu(III)/CTC in the presence of trioctyl phosphine oxide (TOPO) occurs at the H20/CCl4 interface, resulting in greatly enhanced TIR-RLS signals with the maximum peak located at 340 nm. The enhanced TIR-RLS intensity is in proportion to the CTC concentration in the range 0.98 to approximately 20.0 x 10(-7) mol/L. The limit of detection is 9.8 x 10(-9) mol/L. Synthetic samples and body fluid samples including human urine, human serum, and fresh milk were determined with the recovery of 95.4-106.4% and RSD of 2.9-3.9%.

摘要

基于在水/四氯化碳（H₂O/CCl₄）界面处测量全内反射共振光散射（TMR-RLS），提出了一种高选择性测定金霉素（CTC）的方法。在pH值为7.54 - 8.14范围内，在三辛基氧化膦（TOPO）存在下，Eu(III)/CTC二元配合物在H₂O/CCl₄界面发生相互作用，导致全内反射共振光散射信号大幅增强，最大峰值位于340 nm处。增强的全内反射共振光散射强度与0.98至约20.0×10⁻⁷ mol/L范围内的CTC浓度成正比。检测限为9.8×10⁻⁹ mol/L。对合成样品以及包括人尿、人血清和鲜牛奶在内的体液样品进行了测定，回收率为95.4 - 106.4%，相对标准偏差为2.9 - 3.9%。