Zeppa S, Potenza L, Polidori E, Guescini M, Agostini D, Giomaro G, Stocchi V
Istituto di Chimica Biologica Giorgio Fornaini, Università degli Studi di Urbino, Italy.
Curr Genet. 2001 Aug;40(1):49-53. doi: 10.1007/s002940100231.
Ubiquitin is a highly conserved 76-amino acid protein implicated in the function of quite different vital cellular processes. In the present study, we cloned and sequenced a polyubiquitin gene from Tuber borchii (Ubil) that is organised in four tandem repeats, with two C-terminal extension amino acids, serine and leucine. Two introns of 116 bp and 55 bp in length were detected in the first and second repeats, respectively. The Ubil gene is highly expressed in mycelium and is less expressed in the ripe fruiting body. Southern and Northern blot analyses revealed a second form of the ubiquitin gene.
泛素是一种高度保守的由76个氨基酸组成的蛋白质，参与多种不同的重要细胞过程。在本研究中，我们从白块菌中克隆并测序了一个多聚泛素基因（Ubil），该基因由四个串联重复序列组成，C末端有两个延伸氨基酸，丝氨酸和亮氨酸。分别在第一个和第二个重复序列中检测到长度为116 bp和55 bp的两个内含子。Ubil基因在菌丝体中高度表达，在成熟子实体中表达较少。Southern和Northern印迹分析揭示了泛素基因的第二种形式。
