Yoshinaga K, Saxena D K, Oh-oka T, Tanii I, Toshimori K
Department of Anatomy and Reproductive Cell Biology, Miyazaki Medical College, Kiyotake, Miyazaki 889-1692, Japan.
Reproduction. 2001 Oct;122(4):649-55. doi: 10.1530/rep.0.1220649.
The monoclonal antibody mMN9 recognizes an antigenic molecule, equatorin, which is localized at the equatorial segment of the mammalian sperm acrosome. Our previous results using an IVF system indicated that mMN9 blocked sperm-oocyte fusion. Antibody-containing and control solutions were injected directly into the right and left oviductal ampullae, respectively, of anaesthetized female mice to assess the effect of mMN9 on fertilization in vivo. After hCG treatment, the females were mated, and their oviductal eggs and implanted embryos were examined. mMN9 was retained in the oviductal lumen at 20 h after injection. The rates of fertilization and concomitant pregnancy were significantly lower than in the control side (P < 0.05). In addition, histological studies showed no evidence of pathological changes in the female reproductive tract after the injections. These results indicate that mMN9 inhibits mouse fertilization significantly under in vivo conditions and that this injection method should be useful for studying the effects of antibodies and agents on fertilization in vivo.
单克隆抗体mMN9识别一种抗原分子赤道蛋白，该蛋白定位于哺乳动物精子顶体的赤道段。我们先前使用体外受精系统的结果表明，mMN9可阻断精子与卵母细胞的融合。将含抗体溶液和对照溶液分别直接注射到麻醉的雌性小鼠的右侧和左侧输卵管壶腹部，以评估mMN9对体内受精的影响。给予人绒毛膜促性腺激素（hCG）处理后，使雌性小鼠交配，并检查其输卵管内的卵子和着床胚胎。注射后20小时，mMN9保留在输卵管腔内。受精率和同期妊娠率显著低于对照侧（P <0.05）。此外，组织学研究表明注射后雌性生殖道没有病理变化的迹象。这些结果表明，mMN9在体内条件下可显著抑制小鼠受精，并且这种注射方法对于研究抗体和药物对体内受精的影响应该是有用的。
