Chuck R S, Behrens A, Wellik S R, Liaw L H, Sweet P M, Osann K E, McDonnell P J, Berns M W
Department of Ophthalmology, University of California Irvine, Irvine, California 92697, USA.
Lasers Surg Med. 2001;29(3):288-92. doi: 10.1002/lsm.1121.
Most of the in vitro work to characterize the effects of clinical laser surgery on corneal tissues has concentrated on the effects on stromal keratocytes and endothelium with little attention being paid to corneal epithelium. Our purpose is to describe the epithelial healing rates observed in freshly cultured rabbit corneas treated with phototherapeutic keratectomy (PTK).
STUDY DESIGN/MATERIALS AND METHODS: Corneas were placed in a simple organ culture system, with media change every 2 days. A clinical excimer laser was used to perform a 6 mm diameter, 100 microm depth transepithelial PTK on 24 cultured rabbit corneas, 1 day after culture initiation. For each post-treatment day, one experimental and one control cornea were removed from culture and stained with fluorescein, photographed, and fixed for histology. Epithelial defect area was measured with digital imaging software and analyzed statistically to assess the re-epithelialization rate.
Control corneas, maintained in culture for 1-4 days, had no epithelial defects. Those corneas treated with PTK exhibited an immediate epithelial defect that slowly healed over 3 days. This was confirmed on histopathological analysis. A significant linear trend in re-epithelialization across the time points studied was found (F = 80.48, P = 0.0029). The slope of the linear regression model showed an estimate rate of re-epithelialization of -6.70 over the 3 days.
We have described the development of a simple, whole organ, rabbit cornea culture model for re-epithelialization after PTK. Our rates of epithelial healing resemble those found in the literature in live rabbit models. Therefore, this model may possibly be used to monitor epithelial wound healing in different corneal diseases or injuries.
大多数用于描述临床激光手术对角膜组织影响的体外研究工作都集中在对基质角膜细胞和内皮细胞的影响上，而对角膜上皮的关注较少。我们的目的是描述在接受光治疗性角膜切削术（PTK）的新鲜培养兔角膜中观察到的上皮愈合率。
研究设计/材料与方法：将角膜置于简单的器官培养系统中，每2天更换一次培养基。在培养开始1天后，使用临床准分子激光对24只培养的兔角膜进行直径6mm、深度100μm的经上皮PTK。在每个治疗后日，从培养物中取出一只实验角膜和一只对照角膜，用荧光素染色、拍照并固定用于组织学检查。使用数字成像软件测量上皮缺损面积并进行统计分析以评估再上皮化率。
在培养1 - 4天的对照角膜中没有上皮缺损。那些接受PTK治疗的角膜出现了即刻上皮缺损，该缺损在3天内缓慢愈合。这在组织病理学分析中得到了证实。在所研究的时间点上发现再上皮化存在显著的线性趋势（F = 80.48，P = 0.0029）。线性回归模型的斜率显示在3天内再上皮化的估计速率为 - 6.70。
我们描述了一种用于PTK后再上皮化的简单、全器官兔角膜培养模型的建立。我们的上皮愈合率与文献中在活体兔模型中发现的相似。因此，该模型可能可用于监测不同角膜疾病或损伤中的上皮伤口愈合情况。
