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一种在膜片钳记录或离子成像过程中对细胞施加快速加热或冷却刺激的系统。

A system for applying rapid warming or cooling stimuli to cells during patch clamp recording or ion imaging.

作者信息

Reid G, Amuzescu B, Zech E, Flonta M L

机构信息

Department of Animal Physiology and Biophysics, Faculty of Biology, University of Bucharest, Splaiul Independentei 91-95, 76201, Bucharest, Romania.

出版信息

J Neurosci Methods. 2001 Oct 15;111(1):1-8. doi: 10.1016/s0165-0270(01)00416-2.

Abstract

We describe a system for superfusing small groups of cells at a precisely controlled and rapidly adjustable local temperature. Before being applied to the cell or cells under study, solutions are heated or cooled in a chamber of small volume ( approximately 150 microl) and large surface area, sandwiched between four small Peltier elements. The current through the Peltier elements is controlled by a microprocessor using a PID (proportional-integral-derivative) feedback algorithm. The chamber can be heated to at least 60 degrees C and cooled to 0 degrees C, changing its temperature at a maximum rate of about 7 degrees C per second; temperature ramps can be followed under feedback control at up to 4 degrees C per second. Temperature commands can be applied from the digital-to-analogue converter of any laboratory interface or generated digitally by the microprocessor. The peak-to-peak noise contributed by the system does not exceed that contributed by a patch pipette, holder and headstage, making it suitable for single channel as well as whole cell recordings.

摘要

我们描述了一种用于在精确控制且快速可调的局部温度下对小细胞群进行灌流的系统。在应用于所研究的一个或多个细胞之前,溶液在一个体积小(约150微升)且表面积大的腔室中加热或冷却,该腔室夹在四个小型珀耳帖元件之间。通过珀耳帖元件的电流由微处理器使用PID(比例-积分-微分)反馈算法进行控制。该腔室可加热至至少60摄氏度并冷却至0摄氏度,其温度变化的最大速率约为每秒7摄氏度;在反馈控制下,温度斜率可达每秒4摄氏度。温度指令可从任何实验室接口的数模转换器发出,或由微处理器数字生成。该系统产生的峰-峰噪声不超过膜片吸管、固定器和前置放大器产生的噪声,使其适用于单通道以及全细胞记录。

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