Institute of Basic Medical Sciences, Section of Physiology, University of Oslo, Oslo, Norway.
Department of Neurscience, Karolinska Institutet, Solna, Sweden.
Sci Rep. 2024 Nov 18;14(1):28521. doi: 10.1038/s41598-024-79856-4.
Temperature-critical applications, such as patch-clamp electrophysiology, require constant perfusion at a fixed temperature. However, maintaining perfusate at a specific temperature throughout various applications requires heaters or coolers with integrated feedback systems, which has historically increased complexity and cost. This makes such systems prohibitively expensive in research environments with lower funding rates, particularly in developing countries. We developed a custom temperature control system that relies on off-the-shelf components and few custom parts, which can be easily produced with common tools. Our system can be built for less than $30 and maintains a set perfusate temperature within 0.4 °C while introducing negligible electrical interference. Using this system, we demonstrate that Striatal Medium Spiny Neurons exhibit increased membrane resistance, longer membrane time constants, lower firing rates, and increased rheobase current at room temperature compared to physiological temperature.
温度敏感型应用，如膜片钳电生理学，需要在固定温度下持续灌流。然而，要在各种应用中保持灌流液的特定温度，需要带有集成反馈系统的热交换器或冷却器，这在历史上增加了系统的复杂性和成本。这使得此类系统在资金支持率较低的研究环境中过于昂贵，特别是在发展中国家。我们开发了一种依赖现成组件和少量定制部件的定制温度控制系统，这些组件可以使用常见工具轻松制造。我们的系统成本不到 30 美元，可将设定的灌流液温度维持在 0.4°C 以内，同时引入的电干扰可忽略不计。使用该系统，我们证明与生理温度相比，纹状体中间神经元在室温下表现出膜电阻增加、膜时间常数延长、放电率降低和启动电流增加。
