Hara K, Kobayashi M, Akiyama Y
Department of Applied Drug Research, Eisai Co. Ltd., Koishikawa 4-6-10, Bunkyo-ku, Tokyo 112-8088, Japan.
Nihon Yakurigaku Zasshi. 2001 Sep;118(3):231-40. doi: 10.1254/fpj.118.231.
Different types of therapeutic agents for osteoporosis are often simultaneously prescribed for the same patient, but limited experimental findings indicate the significance of combined treatment. In the present study, the inhibitory effect of combined vitamin K2(K2) and 1 alpha-(OH)-vitamin D3(D3) treatment on bone loss was compared to that of K2 or D3 alone in ovariectomized rats. Female rats (20-week-old) were ovariectomized and divided into 4 groups as follows: they were treated for 8 weeks with vehicle, K2 (30 mg/kg), D3 (0.3 microgram/kg) and K2 and D3 (K2 + D3) at the respective doses. K2 was given as a dietary supplement and D3 was orally administered 3 times a week. Bone density of the femurs was measured by peripheral quantitative computed tomography. Ovariectomy resulted in decreased bone density in proximal metaphysis, especially in the trabecular region, and treatment with K2, D3 or K2 + D3 inhibited this decrease. Moreover, in the K2 + D3 group, bone density and mineral content in the trabecular region in proximal metaphysis and cortical bone width in diaphysis were significantly higher than those in the D3 group. Consistent with these observations, bone strength in the femoral midshaft tended to increase only in the K2 + D3 group compared to that in the vehicle group. These findings indicate that combined K2 and D3 treatment is more effective for bone loss than that with K2 or D3 alone.
治疗骨质疏松症的不同类型治疗药物常常会同时开给同一位患者，但有限的实验结果表明联合治疗的重要性。在本研究中，将维生素K2（K2）与1α-羟基维生素D3（D3）联合治疗对去卵巢大鼠骨质流失的抑制作用与单独使用K2或D3的作用进行了比较。将20周龄的雌性大鼠去卵巢，并分为以下4组：分别用赋形剂、K2（30毫克/千克）、D3（0.3微克/千克）以及K2和D3（K2 + D3）以各自的剂量治疗8周。K2作为膳食补充剂给予，D3每周口服给药3次。通过外周定量计算机断层扫描测量股骨的骨密度。去卵巢导致近端干骺端骨密度降低，尤其是在小梁区域，而用K2、D3或K2 + D3治疗可抑制这种降低。此外，在K2 + D3组中，近端干骺端小梁区域的骨密度和矿物质含量以及骨干皮质骨宽度均显著高于D3组。与这些观察结果一致，与赋形剂组相比，仅K2 + D3组股骨中轴的骨强度有增加的趋势。这些发现表明，K2和D3联合治疗对骨质流失的效果比单独使用K2或D3更有效。
