Varedi M, Lee H M, Greeley G H, Herndon D N, Englander E W
Department of Surgery and Shriners Hospitals for Children, The University of Texas Medical Branch, Galveston 77555, USA.
Shock. 2001 Oct;16(4):259-63. doi: 10.1097/00024382-200116040-00004.
Severe burn injury is commonly associated with significant changes in intestinal epithelial function. These changes include mucosal atrophy and increased permeability. To date, the mechanism by which burn injury alters intestinal epithelium function are not clear. We used an in vitro model of intestinal epithelium, IEC-6 cells, and observed that the integrity of confluent culture is disrupted and cell growth and migration rates are reduced in the presence of serum collected from scald burn injury rats (SRS) (6). To identify gene products involved in mechanisms underlying these effects, we used the cDNA expression microarray analysis and found that genes whose expression was affected by SRS in IEC-6 cells were primarily associated with cell shape, growth and death, stress-response, protein turnover and transport of water and electrolytes. These data demonstrate that a burn-induced circulating factor(s) modulates expression of genes, which may affect intestinal epithelial cell survival and function. Thus, these findings provide clues to the nature of molecular mechanisms potentially involved in multiple-organ malfunction, in particular the atrophy and enhanced permeability of gut mucosa, after burn injury.
严重烧伤通常与肠上皮功能的显著变化相关。这些变化包括粘膜萎缩和通透性增加。迄今为止，烧伤损伤改变肠上皮功能的机制尚不清楚。我们使用了一种肠上皮的体外模型，即IEC-6细胞，并观察到在从烫伤大鼠收集的血清（SRS）存在的情况下，汇合培养的完整性被破坏，细胞生长和迁移率降低（6）。为了鉴定参与这些效应潜在机制的基因产物，我们使用了cDNA表达微阵列分析，发现IEC-6细胞中受SRS影响表达的基因主要与细胞形状、生长和死亡、应激反应、蛋白质周转以及水和电解质转运有关。这些数据表明，烧伤诱导的循环因子调节基因表达，这可能影响肠上皮细胞的存活和功能。因此，这些发现为烧伤损伤后多器官功能障碍，特别是肠粘膜萎缩和通透性增强所潜在涉及的分子机制的性质提供了线索。
