Jakubíková J, Duraj T, Takácsová X, Hunáková L, Chorváth B, Sedlák J
Cancer Research Institute, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Neoplasma. 2001;48(3):208-13.
The cytotoxic activity of the non-steroidal anti-inflammatory agent ibuprofen to human promyelocytic leukemia cell line HL-60, its multidrug-resistant subline HL-60/VCR (MDR-1 gene coded P-glycoprotein), as well as myeloma U266 and B-lymphoblastoid ARH-77 cell lines was demonstrated with the aid of flow cytometric analysis. Ibuprofen inhibited proliferation and induced apoptosis (detected as sub-G0 nuclei, fluorescein diacetate staining, Annexin-V binding cells and agarose electrophoretic detection of nucleosomal DNA fragmentation) in promyelocytic cells and, to a lesser extent, in U266 and ARH-77 cells.
借助流式细胞术分析，证实了非甾体抗炎药布洛芬对人早幼粒细胞白血病细胞系HL-60、其多药耐药亚系HL-60/VCR（MDR-1基因编码的P-糖蛋白）以及骨髓瘤U266和B淋巴细胞样ARH-77细胞系具有细胞毒性活性。布洛芬抑制早幼粒细胞的增殖并诱导其凋亡（通过亚G0期细胞核、荧光素二乙酸酯染色、膜联蛋白V结合细胞以及核小体DNA片段的琼脂糖电泳检测来检测），对U266和ARH-77细胞的抑制作用较小。
