Stanford W L, Cohn J B, Cordes S P
Programme in Development and Fetal Health, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Room 983, Toronto, Ontario, Canada M5G 1X5.
Nat Rev Genet. 2001 Oct;2(10):756-68. doi: 10.1038/35093548.
Although at least 35,000 human genes have been sequenced and mapped, adequate expression or functional information is available for only approximately 15% of them. Gene-trap mutagenesis is a technique that randomly generates loss-of-function mutations and reports the expression of many mouse genes. At present, several large-scale, gene-trap screens are being carried out with various new vectors, which aim to generate a public resource of mutagenized embryonic stem (ES) cells. This resource now includes more than 8,000 mutagenized ES-cell lines, which are freely available, making it an appropriate time to evaluate the recent advances in this area of genomic technology and the technical hurdles it has yet to overcome.
尽管至少35000个人类基因已被测序和定位，但只有约15%的基因有足够的表达或功能信息。基因捕获诱变是一种随机产生功能丧失突变并报告许多小鼠基因表达的技术。目前，正在使用各种新载体进行几项大规模的基因捕获筛选，目的是生成诱变胚胎干细胞（ES细胞）的公共资源。该资源现在包括8000多个诱变ES细胞系，这些细胞系可免费获取，这使得现在是评估这一基因组技术领域的最新进展及其尚未克服的技术障碍的适当时机。
