在小鼠中构建染色体重排

Engineering chromosomal rearrangements in mice.

作者信息

Yu Y, Bradley A

机构信息

Program in Developmental Biology, Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030,

出版信息

Nat Rev Genet. 2001 Oct;2(10):780-90. doi: 10.1038/35093564.

DOI:10.1038/35093564

PMID:11584294

Abstract

The combination of gene-targeting techniques in mouse embryonic stem cells and the Cre/loxP site-specific recombination system has resulted in the emergence of chromosomal-engineering technology in mice. This advance has opened up new opportunities for modelling human diseases that are associated with chromosomal rearrangements. It has also led to the generation of visibly marked deletions and balancer chromosomes in mice, which provide essential reagents for maximizing the efficiency of large-scale mutagenesis efforts and which will accelerate the functional annotation of mammalian genomes, including the human genome.

摘要

小鼠胚胎干细胞中的基因靶向技术与Cre/loxP位点特异性重组系统相结合，催生了小鼠染色体工程技术。这一进展为模拟与染色体重排相关的人类疾病带来了新机遇。它还促使小鼠中出现了明显标记的缺失和平衡染色体，这些染色体为提高大规模诱变效率提供了重要试剂，也将加速包括人类基因组在内的哺乳动物基因组的功能注释。