大鼠纹状体中间神经元中神经元型一氧化氮合酶与神经激肽-1受体的共定位

Colocalization of neuronal nitric oxide synthase and neurokinin-1 receptor in striatal interneurons in the rat.

作者信息

Li J L, Kaneko T, Mizuno N

机构信息

Department of Anatomy and K. K. Leung Brain Research Center, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.

出版信息

Neurosci Lett. 2001 Sep 14;310(2-3):109-12. doi: 10.1016/s0304-3940(01)02097-3.

DOI:10.1016/s0304-3940(01)02097-3

PMID:11585579

Abstract

It has been established that nitric oxide synthase (NOS)-containing aspiny neurons constitute one class of interneurons in the striatum, and that substance P (SP)-containing projection neurons give off many axon collaterals within the striatum. In the present study, we investigated a morphological substrate of possible influences of SP-containing projection neurons upon NOS-containing interneuron in the rat striatum; colocalization of immunoreactivities for NOS and NK-1 type tachykinin receptor (NK1R: SP receptor) was examined by a double-immunofluorescence histochemistry. The vast majority (94.6%) of NOS-positive neurons showed NK1R immunoreactivity, whereas only smaller cells of NK1R-positive neurons (61.2% of NK1R-positive neurons) displayed NOS immunoreactivity. The results indicated that the NOS-containing interneurons were under direct control of SP-containing projection neurons in the striatum.

摘要

已经确定，含一氧化氮合酶（NOS）的无棘神经元构成纹状体中的一类中间神经元，并且含P物质（SP）的投射神经元在纹状体内发出许多轴突侧支。在本研究中，我们研究了含SP的投射神经元对大鼠纹状体中含NOS的中间神经元可能产生影响的形态学基础；通过双重免疫荧光组织化学检查了NOS和NK-1型速激肽受体（NK1R：SP受体）免疫反应性的共定位。绝大多数（94.6%）NOS阳性神经元显示NK1R免疫反应性，而只有较小的NK1R阳性神经元（占NK1R阳性神经元的61.2%）显示NOS免疫反应性。结果表明，含NOS的中间神经元受纹状体内含SP的投射神经元的直接控制。