Li Jin-Lian, Kaneko Takeshi, Mizuno Noboru
Department of Anatomy and K.K. Leung Brain Research Center, The Fourth Military Medical University, Xi'an 710032, People's Republic of China.
Neurosci Lett. 2002 May 10;324(1):9-12. doi: 10.1016/s0304-3940(02)00138-6.
We examined if axon terminals of dopaminergic neurons might make synapses upon neurokinin-1 receptor (NK1R)-expressing intrinsic neurons in the rat striatum. In a double-immunocytochemical ultrastructural study, dopaminergic terminals were labeled by the immunoperoxidase method for tyrosine hydroxylase (TH), while NK1R-immunoreactivity (-IR) was revealed by the immunogold-silver labeling method. Some TH-immunoreactive (-ir) axon terminals formed synapses of the symmetric or intermediate type on NK1R-ir neuronal profiles; usually on dendritic profiles and rarely on somatic profiles. It was further confirmed by means of triple-immunofluorescence histochemistry that NK1R-ir neurons in close association with TH-ir axon terminals showed nitric oxide synthase (NOS)- or vesicular acetylcholine transporter-IR. Since NK1R-expressing striatal neurons are segregated into cholinergic and somatostatin/NOS-containing intrinsic neurons (Brain Res. 631 (1993) 297; Neurosci. Lett. 310 (2001) 109), the present results indicate that dopaminergic neurons make synapses upon these intrinsic neurons in the striatum.
我们研究了多巴胺能神经元的轴突终末是否会与大鼠纹状体内表达神经激肽-1受体（NK1R）的内在神经元形成突触。在一项双重免疫细胞化学超微结构研究中，多巴胺能终末通过酪氨酸羟化酶（TH）的免疫过氧化物酶法进行标记，而NK1R免疫反应性（-IR）则通过免疫金银标记法显示。一些TH免疫反应性（-ir）轴突终末在NK1R-ir神经元轮廓上形成对称或中间型突触；通常在树突轮廓上，很少在体细胞轮廓上。通过三重免疫荧光组织化学进一步证实，与TH-ir轴突终末紧密相关的NK1R-ir神经元显示出一氧化氮合酶（NOS）或囊泡乙酰胆碱转运体-IR。由于表达NK1R的纹状体神经元被分为胆碱能和含生长抑素/NOS的内在神经元（《脑研究》631（1993）297；《神经科学快报》310（2001）109），目前的结果表明多巴胺能神经元在纹状体中的这些内在神经元上形成突触。
