Ellington J J, Evans J J, Prickett K B, Champion W L
US Environmental Protection Agency, National Exposure Research Laboratory, Ecosystems Research Division, Athens, GA 30605, USA.
J Chromatogr A. 2001 Sep 14;928(2):145-54. doi: 10.1016/s0021-9673(01)01138-4.
High-performance liquid chromatographic separation of the individual enantiomers of 12 organophosphorus pesticides (OPs) was obtained on polysaccharide enantioselective HPLC columns using alkane-alcohol mobile phase. The OP pesticides were crotoxyphos, dialifor, fonofos, fenamiphos, fensulfothion, isofenphos, malathion, methamidophos, profenofos, crufomate, prothiophos and trichloronate. The enantiomers of fenamiphos, fensulfothion, profenofos and crufomate were separated on CHIRALPAK AD; the enantiomers of fenamiphos were also separated on CHIRALPAK AS; the enantiomers of methamidophos, crufomate and trichloronate were separated on CHIRALCEL OD; the enantiomers of crotoxyphos, dialifor, fonofos, malathion, prothiophos and trichloronate were separated on CHIRALCEL OJ; and the enantiomers of isofenphos were separated on CHIRALCEL OG. Baseline or partial separation of the enantiomers of six of these OP pesticides was obtained on CHIRALCEL OJ. In continued method development, the separation of the enantiomers of the 12 OPs was investigated more extensively on CHIRALCEL OJ to determine whether the mobile phase composition, flow-rate and column temperature could be optimized to yield at least partial separation of the enantiomers. Chromatographic conditions were found that gave either baseline or near baseline separations of the enantiomers of the 12 OPs on the CHIRALCEL OJ column.
使用烷烃 - 醇流动相，在多糖对映体选择性高效液相色谱柱上实现了12种有机磷农药（OPs）各对映体的高效液相色谱分离。这些OP农药包括巴毒磷、毒虫畏、地虫硫磷、苯线磷、甲拌磷、异柳磷、马拉硫磷、甲胺磷、丙溴磷、育畜磷、丙硫磷和毒壤磷。苯线磷、甲拌磷、丙溴磷和育畜磷的对映体在CHIRALPAK AD柱上分离；苯线磷的对映体也在CHIRALPAK AS柱上分离；甲胺磷、育畜磷和毒壤磷的对映体在CHIRALCEL OD柱上分离；巴毒磷、毒虫畏、地虫硫磷、马拉硫磷、丙硫磷和毒壤磷的对映体在CHIRALCEL OJ柱上分离；异柳磷的对映体在CHIRALCEL OG柱上分离。在CHIRALCEL OJ柱上实现了其中6种OP农药对映体的基线或部分分离。在后续的方法开发中，对CHIRALCEL OJ柱上12种OPs对映体的分离进行了更广泛的研究，以确定流动相组成、流速和柱温是否可以优化，以实现对映体的至少部分分离。结果发现了色谱条件，在CHIRALCEL OJ柱上实现了12种OPs对映体的基线或接近基线分离。
