Hassell J R, Newsome D A, Hascall V C
J Biol Chem. 1979 Dec 25;254(24):12346-54.
The proteoglycans of the Rhesus monkey corneal stroma were characterized by analyzing both radiolabeled proteoglycans synthesized by corneas in organ culture and native corneal proteoglycans obtained by large scale preparations. The analyses indicate that the proteoglycans synthesized in organ culture were similar to, if not identical with, their counterparts in the stroma although they are synthesized in different prportions in vitro than they acumulate in vivo. The corneal stroma contains two proteoglycans. The chondroitin-dermatan sulfate proteoglycan consists of approximately 70% protein and has a Mr = approximately 100,000 to 150,000. It contains one chondroitin-dermatan sulfate side chain of Mr = approximately 55,000. The keratan sulfate proteoglycan consists of approximately 74% protein and has a Mr = approximately -40,000 to 70,000. It contains one or two keratan sulfate side chains with a Mr = approximately 7,000 each. Radiolabeling indicates that both proteoglycans contain glycoprotein-type oligosaccharides as part of their structure.
通过分析器官培养中角膜合成的放射性标记蛋白聚糖和大规模制备获得的天然角膜蛋白聚糖，对恒河猴角膜基质的蛋白聚糖进行了表征。分析表明，尽管在体外合成的比例与在体内积累的比例不同，但器官培养中合成的蛋白聚糖与基质中的对应物相似，即便不是完全相同。角膜基质含有两种蛋白聚糖。硫酸软骨素 - 硫酸皮肤素蛋白聚糖约由70%的蛋白质组成，分子量约为100,000至150,000。它含有一条分子量约为55,000的硫酸软骨素 - 硫酸皮肤素侧链。硫酸角质素蛋白聚糖约由74%的蛋白质组成，分子量约为40,000至70,000。它含有一条或两条分子量各约为7,000的硫酸角质素侧链。放射性标记表明，这两种蛋白聚糖都含有糖蛋白型寡糖作为其结构的一部分。
