Christie W W
J Chromatogr Sci. 1975 Sep;13(9):411-5. doi: 10.1093/chromsci/13.9.411.
The stereospecific analysis of triglycerides is a complex, multistep procedure and some losses inevitably occur at every stage. Such losses are particularly important when the triglycerides contain isotopically-labelled fatty acids incorporated by incubation in vitro with the relevant enzyme systems, but they can be compensated for by adding, prior to the analysis, a triglyceride containing only the same labelled odd-chain fatty acid in each position.
甘油三酯的立体特异性分析是一个复杂的多步骤过程,在每个阶段都不可避免地会有一些损失。当甘油三酯含有通过与相关酶系统体外孵育掺入的同位素标记脂肪酸时,这些损失尤为重要,但可以在分析前添加一种在每个位置仅含有相同标记奇数链脂肪酸的甘油三酯来进行补偿。