Romero-Piffiguer M D, Riera C M
J Immunol Methods. 1979;30(2):153-9. doi: 10.1016/0022-1759(79)90089-9.
This paper describes an extremely simple method for the purification of secretory IgA in two steps from a colostrum pool. The first step of this technique utilizes the agar transparency effect produced by high concentrations of secretory IgA contained in colostrum pool when it is subjected to electrophoresis in this support. The secretory IgA is obtained by extraction and elution of the transparent zone adjacent to the well. In the second step, in order to obtain the maximum degree of purity, the eluate is subjected to precipitation with ammonium sulfate (1.33 M) or chromatographed through Sephadex G-200 gel. The simplicity of the method and the purity of the protein obtained make this procedure suitable for purification of macromolecular substances in concentrated state.
本文描述了一种从初乳池中分两步纯化分泌型IgA的极其简单的方法。该技术的第一步利用了初乳池中高浓度分泌型IgA在这种支持物上进行电泳时产生的琼脂透明效应。通过提取和洗脱孔附近的透明区获得分泌型IgA。在第二步中，为了获得最大程度的纯度，将洗脱液用硫酸铵（1.33 M）沉淀或通过Sephadex G-200凝胶进行色谱分离。该方法的简单性以及所获得蛋白质的纯度使得该程序适用于浓缩状态下大分子物质的纯化。
