Takamiya H, Batsford S R, Tokunaga J, Vogt A
J Immunol Methods. 1979;30(3):277-88. doi: 10.1016/0022-1759(79)90102-9.
Glycol methacrylate-Quetol 523, introduced by Kushida (1977) for combined light and electronmicroscopy studies at low magnification, also permits application of immunofluorescence methods to semithin sections. To recover the antigenicity of proteins fixed with formaldehyde, abrupt dehydration before embedding and subsequent treatment of the semithin sections with protease were essential. Post-staining with suitable histological stains allows exact correlation of antigen localization with tissue structure.
甲基丙烯酸乙二醇酯-喹托523由串田（1977年）推出，用于低倍放大下的光镜与电镜联合研究，也可将免疫荧光方法应用于半薄切片。为恢复用甲醛固定的蛋白质的抗原性，包埋前的快速脱水以及随后用蛋白酶处理半薄切片至关重要。用合适的组织学染色剂进行复染可使抗原定位与组织结构精确对应。
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