包埋前免疫组织化学作为一种在光学显微镜水平进行高分辨率抗原定位的方法。

Pre-embedding immunohistochemistry as an approach to high resolution antigen localization at the light microscopic level.

作者信息

Franklin R M, Martin M T

出版信息

Histochemistry. 1981;72(2):173-90. doi: 10.1007/BF00517131.

Abstract

Pre-embedding immunohistochemistry with subsequent embedding in hydroxypropyl methacrylate enables one to obtain high resolution staining of antigens in 1 mu tissue sections. A routine method using formaldehyde fixation, methanol permeation, and an indirect method with fluorescein-labeled second antibody is described. This method is compared with other pre-embedding staining procedures. To illustrate the method the mouse small intestine was chosen as a model and stained with antibodies to tubulin, actin, and fibronectin. Some anticipated and some unusual staining patterns were found.

摘要

预包埋免疫组织化学，随后包埋于甲基丙烯酸羟丙酯中，能够在1微米的组织切片中获得抗原的高分辨率染色。本文描述了一种使用甲醛固定、甲醇渗透的常规方法，以及一种使用荧光素标记二抗的间接方法。该方法与其他预包埋染色程序进行了比较。为了说明该方法，选择小鼠小肠作为模型，并用抗微管蛋白、肌动蛋白和纤连蛋白的抗体进行染色。发现了一些预期的和一些不寻常的染色模式。