内源性脑源性神经营养因子在双侧视觉剥夺后上丘长期神经元重组中的潜在作用。

Potential role of endogenous brain-derived neurotrophic factor in long-term neuronal reorganization of the superior colliculus after bilateral visual deprivation.

作者信息

Vizuete M L, Venero J L, Vargas C, Revuelta M, Machado A, Cano J

机构信息

Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, 41012, Spain.

出版信息

Neurobiol Dis. 2001 Oct;8(5):866-80. doi: 10.1006/nbdi.2001.0424.

DOI:10.1006/nbdi.2001.0424

PMID:11592854

Abstract

The role of the brain-derived neurotrophic factor (BDNF), the BDNF receptor (TrkB), and the glutamic acid decarboxylase (GAD67) after neonatal, bilateral nerve deafferentiation during postnatal development was investigated in the rat superior colliculus (SC). BDNF and GAD67 mRNA expression were significantly increased in optic (Op) and intermediate gray (InG) layers at 5, 8, 15, and 21 days after birth, but not in adult animals. However, TrkB mRNA expression was not modified at any time tested. At 15 days, where changes in BDNF and GAD67 mRNAs were more evident, an upregulation of the NMDAR(1A) mRNA glutamate receptor in the Op and InG, a modification in the pattern of synaptic zinc in the superficial layers of SC, and an increased synaptophysin immunoreactivity in the Op was found. This indicates the existence of a synergic mechanism between BDNF and NMDA to determine refinement of connections after the loss of visual input in SC.

摘要

研究了大鼠上丘（SC）在出生后发育过程中经历新生期双侧神经去传入后，脑源性神经营养因子（BDNF）、BDNF受体（TrkB）和谷氨酸脱羧酶（GAD67）的作用。出生后5天、8天、15天和21天，视（Op）层和中间灰质（InG）层中BDNF和GAD67 mRNA表达显著增加，但成年动物中未增加。然而，在任何测试时间，TrkB mRNA表达均未改变。在15天时，BDNF和GAD67 mRNA的变化更为明显，此时发现Op层和InG层中NMDAR(1A) mRNA谷氨酸受体上调、SC浅层突触锌模式改变以及Op层中突触素免疫反应性增加。这表明BDNF和NMDA之间存在协同机制，以确定SC视觉输入丧失后连接的精细化。