[转化生长因子-β与α-平滑肌肌动蛋白在瘢痕中的表达及相关性研究]
[A study on the expressions and the correlation of TGF-beta and alpha-SMA in scars].
作者信息
Tian Y, Tang S, Luo S
机构信息
Department of Burns and Plastic Surgery, Guangdong Provincial Corps Hospital, Chinese People's Armed Police Forces, Guangzhou 510507, PR China.
出版信息
Zhonghua Zheng Xing Wai Ke Za Zhi. 2000 Mar;16(2):75-7.
OBJECTIVE
To detect the expressions of TGF-beta(TGF-beta 1, TGF-beta 2, TGF-beta 3) and alpha-SMA and to investigate the probable effects of TGF-beta in the formation of hypertrophic scars as well as the expression of alpha-SMA in the scars.
METHODS
28 samples of hypertrophic scars, 19 samples of flat scars and 16 samples of normal skin have been examined immunohistochemically for the expressions of TGF-beta and alpha-SMA. Then a statistical and correlative analysis was carried out among the groups based on patient age, sex and scarring time.
RESULTS
- TGF-beta and alpha-SMA have no expression in normal skin, but have varied expressions in hypertrophic scars and flat scars. 2. TGF-beta 1, TGF-beta 2 and alpha-SMA expressed highly in hypertrophic scar in comparison with normal skin and flat scar with a significantly statistical difference (P < 0.01). 3. There was a high expression of TGF-beta 3 in the flat scar and some hypertrophic scar that were from the female group or the scar over one year. 4. There was no statistical difference between the groups of teenage and adults. 5. The correlative analysis of TGF-beta to alpha-SMA demonstrated that the algebraic value of TGF-beta 1 + TGF-beta 2 - TGF-beta 3 correlates positively to alpha-SMA (r = 0.57). TGF-beta 3 correlates negatively to alpha-SMA(r = -0.80).
CONCLUSIONS
- The formation and contraction of scar result from the synergic effects of TGF-beta 1, TGF-beta 2 and TGF-beta 3. TGF beta 1, TGF-beta 2 correlate the hyperplasia and contraction of scar, whereas TGF-beta 3 correlates the maturing of scar. To suppress the expression of TGF-beta 1, TGF-beta 2 or promote the expression of TGF-beta 3 would be beneficial to prevention of hyperplasia and contraction of scar. 2. It is reasonable to postulate that the female have stronger resistance to scar hyperplasia in that they have stronger expression of TGF-beta 3, which deserves further clinical observation and investigation. 3. The age has no influence on the expression of TGF-beta and alpha-SMA.
目的
检测转化生长因子β（TGF-β，包括TGF-β1、TGF-β2、TGF-β3）及α平滑肌肌动蛋白（α-SMA）的表达，探讨TGF-β在增生性瘢痕形成中的可能作用以及α-SMA在瘢痕中的表达情况。
方法
采用免疫组织化学方法检测28例增生性瘢痕、19例扁平瘢痕及16例正常皮肤组织中TGF-β及α-SMA的表达。然后根据患者年龄、性别及瘢痕形成时间在各研究组间进行统计学及相关性分析。
结果
- TGF-β及α-SMA在正常皮肤中无表达，但在增生性瘢痕及扁平瘢痕中有不同程度表达。2. 与正常皮肤及扁平瘢痕相比，TGF-β1、TGF-β2及α-SMA在增生性瘢痕中高表达，差异有统计学意义（P<0.01）。3. TGF-β3在女性患者或病程超过1年的扁平瘢痕及部分增生性瘢痕中有高表达。4. 青少年组与成人组之间差异无统计学意义。5. TGF-β与α-SMA的相关性分析显示，TGF-β1+TGF-β2-TGF-β3的代数和与α-SMA呈正相关（r=0.57），TGF-β3与α-SMA呈负相关（r=-0.80）。
结论
- 瘢痕的形成与收缩是TGF-β1、TGF-β2及TGF-β3协同作用的结果。TGF-β1、TGF-β2与瘢痕的增生及收缩相关，而TGF-β3与瘢痕的成熟相关。抑制TGF-β1、TGF-β2的表达或促进TGF-β3的表达将有助于预防瘢痕增生及收缩。2. 推测女性对瘢痕增生的抵抗力较强，因其TGF-β3表达较强，这值得进一步临床观察及研究。3. 年龄对TGF-β及α-SMA的表达无影响。
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