Zhang Y, Huber M, Euler-König I, Süssmuth R, Jung G, Jassoy C
Institute for Virology and Immunobiology, Medical Policlinic, University of Würzburg, Versbacher Strasse 7, 97078 Würzburg, Germany.
Immunol Lett. 2001 Nov 1;79(1-2):93-6. doi: 10.1016/s0165-2478(01)00270-x.
Proliferative responses to recombinant HIV proteins in infected individuals may represent a correlate of protection from disease progression. In this study, the proliferative responses to HIV p24, p55 and gp120 were evaluated in infected subjects. Whereas, vigorous proliferative responses directed at the Gag proteins were detected in several individuals, Env-specific proliferation was observed in only one subject. Epitope mapping using overlapping peptides demonstrated proliferative responses of PBMC to Gag peptides. Responses were broadly directed at multiple peptides in some subjects. Although several of the peptides that induced proliferative responses also contain CTL epitopes potentially relevant in the particular individuals, many additional Gag T cell epitopes were present in each subject. This finding may be relevant for the design and testing of HIV candidate vaccines.
感染个体对重组HIV蛋白的增殖反应可能代表了预防疾病进展的一个相关因素。在本研究中，对感染受试者针对HIV p24、p55和gp120的增殖反应进行了评估。然而，在数名个体中检测到了针对Gag蛋白的强烈增殖反应，仅在一名受试者中观察到Env特异性增殖。使用重叠肽进行的表位作图显示PBMC对Gag肽有增殖反应。在一些受试者中，反应广泛针对多种肽。虽然一些诱导增殖反应的肽也含有在特定个体中可能相关的CTL表位，但每个受试者中还存在许多额外的Gag T细胞表位。这一发现可能与HIV候选疫苗的设计和测试相关。
