Debreceni A, Okazaki K, Matsushima Y, Ohana M, Nakase H, Uchida K, Uose S, Chiba T
Department of Gastroenterology and Hepatology, Faculty of Medicine, Kyoto University, Shogoin-Kawaharamachi, Sakyo-ku, 606 Kyoto, Japan.
J Physiol Paris. 2001 Jan-Dec;95(1-6):461-7. doi: 10.1016/s0928-4257(01)00064-x.
A group of the proinflammatory and chemotactic cytokines (chemokines) has been considered as an important factor in the pathomechanism of different bacterial diseases, among them the common Helicobacter pylori infection. Experimental results obtained with gastric biopsy samples of H. pylori positive patients, and with H. pylori infected tumor originated gastric cell lines indicated that these cytokines have essential roles in the development and maintenance of the immune response and inflammation of the gastric mucosa during H. pylori infection. Although the mRNA expression was shown in these biopsy samples and cell lines, it is not yet proved that the normal gastric mucosal epithelial cells themselves express these cytokines. The establishment of a gastric surface mucous cell line with non-tumor origin (GSM06), and the usage of Helicobacter felis as a model of the classic H. pylori infection gave us the possibility to check this question.
in this study GSM06 cells were infected with different numbers (10(5), 10(6), 10(7), 10(8), 10(9) bacterium/ml medium) of H. felis for two different time periods (2, 4 h). Cells treated with medium only were used as control. Then the mRNA expression of the following cytokines was measured by RT-PCR method in the GSM06 cells: proinflammatory cytokine IL1-beta, and chemokine RANTES, eotaxin, MCP-1, MIP1-alpha and MIP1-beta.
we found that neither mRNA of the investigated cytokines was expressed constitutively, however the GSM06 cells expressed the mRNA of each cytokine during H. felis infection.
our results prove that normal gastric surface mucous epithelial cells express immunologically active peptides during H. felis infection. We may suppose that the epithelial cells of the gastric mucosa contribute to the immune response and inflammation by expressing proinflammatory (IL1-beta) and chemotactic (RANTES, eotaxin, MCP-1, MIP1-alpha and beta) cytokines during H. pylori infection in human.
一组促炎和趋化细胞因子（趋化因子）被认为是不同细菌性疾病发病机制中的重要因素，其中包括常见的幽门螺杆菌感染。对幽门螺杆菌阳性患者的胃活检样本以及幽门螺杆菌感染的肿瘤源性胃细胞系进行的实验结果表明，这些细胞因子在幽门螺杆菌感染期间胃黏膜免疫反应和炎症的发生及维持中起着重要作用。尽管在这些活检样本和细胞系中显示有mRNA表达，但尚未证实正常胃黏膜上皮细胞自身能表达这些细胞因子。建立非肿瘤源性的胃表面黏液细胞系（GSM06）以及使用猫幽门螺杆菌作为经典幽门螺杆菌感染的模型，使我们有机会检验这个问题。
在本研究中，GSM06细胞用不同数量（10⁵、10⁶、10⁷、10⁸、10⁹个细菌/毫升培养基）的猫幽门螺杆菌感染两个不同时间段（2、4小时）。仅用培养基处理的细胞用作对照。然后通过RT-PCR法在GSM06细胞中检测以下细胞因子的mRNA表达：促炎细胞因子IL1-β以及趋化因子RANTES、嗜酸性粒细胞趋化蛋白、MCP-1、MIP1-α和MIP1-β。
我们发现所研究的细胞因子的mRNA均无组成性表达，然而GSM06细胞在猫幽门螺杆菌感染期间表达了每种细胞因子的mRNA。
我们的结果证明，正常胃表面黏液上皮细胞在猫幽门螺杆菌感染期间表达具有免疫活性的肽。我们可以推测，在人类幽门螺杆菌感染期间，胃黏膜上皮细胞通过表达促炎（IL1-β）和趋化（RANTES、嗜酸性粒细胞趋化蛋白、MCP-1、MIP1-α和β）细胞因子来促进免疫反应和炎症。
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