幽门螺杆菌感染患者黏膜细胞因子mRNA的表达

Expression of mucosal cyto-chemokine mRNAs in patients with Helicobacter pylori infection.

作者信息

Park S M, Kim J H, Hong Y H, Jung H R, Park J, Kim J G, Yoo B C

机构信息

Department of Internal Medicine, College of Medicine, Chungang University, Seoul, Korea.

出版信息

Korean J Intern Med. 2001 Dec;16(4):230-5. doi: 10.3904/kjim.2001.16.4.230.

DOI:10.3904/kjim.2001.16.4.230

PMID:11855151

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4578057/

Abstract

BACKGROUND

Helicobacter pylori-induced destruction of the gastroduodenal mucosal barrier is initiated with mucosal infiltration of inflammatory cells. Cytokines and chemokines have been suggested to play important roles in the migration and activation of these inflammatory cells into the mucosa. The present study aimed to investigate expression rates of cyto-chemokine mRNAs using gastric mucosal biopsy specimens.

METHODS

In 98 patients infected with Helicobacter pylori, mucosal mRNA expression rates of cytokines (IL-1 beta, IL-6, and IL-10), C-C chemokines (macrophage inflammatory protein 1 alpha (MIP-1 alpha), and macrophage inflammatory protein 1 beta (MIP-1 beta), monocyte chemotactic and activating factor (MCAF), regulated on activation, normal T cell expressed and presumably secreted (RANTES)) and C-X-C chemokines (IL-8 and growth regulated a (GRO-alpha)) were examined using reverse transcription polymerase chain reaction (RT-PCR).

RESULTS

The expression rates of mRNA for IL-8, GRO-alpha, MIP-1 alpha and RANTES were significantly more increased in H. pylori-positive patients than in H. pylori-negative patients. However, the expressions of IL-1 beta, IL-6 and IL-10 mRNA were statistically not different between two groups. After eradication of H. pylori, expressions of mRNA for three cytokines (IL-1 beta, IL-6 and IL-10), four C-C chemokines (MIP-1 alpha, MIP-1 beta, MCAF and RANTES) and two C-X-C chemokines (IL-8 and GRO-alpha) were significantly decreased.

CONCLUSION

These results suggest that C-X-C chemokines and some C-C chemokines play important roles in H. pylori-associated peptic ulcer diseases.

摘要

背景

幽门螺杆菌引起的胃十二指肠黏膜屏障破坏始于炎性细胞的黏膜浸润。细胞因子和趋化因子被认为在这些炎性细胞向黏膜的迁移和激活中起重要作用。本研究旨在使用胃黏膜活检标本调查细胞趋化因子mRNA的表达率。

方法

在98例幽门螺杆菌感染患者中，使用逆转录聚合酶链反应（RT-PCR）检测细胞因子（IL-1β、IL-6和IL-10）、C-C趋化因子（巨噬细胞炎性蛋白1α（MIP-1α）、巨噬细胞炎性蛋白1β（MIP-1β）、单核细胞趋化和激活因子（MCAF）、活化调节正常T细胞表达和可能分泌因子（RANTES））以及C-X-C趋化因子（IL-8和生长调节因子α（GRO-α））的黏膜mRNA表达率。

结果

幽门螺杆菌阳性患者中IL-8、GRO-α、MIP-1α和RANTES的mRNA表达率显著高于幽门螺杆菌阴性患者。然而，两组之间IL-1β、IL-6和IL-10 mRNA的表达在统计学上无差异。根除幽门螺杆菌后，三种细胞因子（IL-1β、IL-6和IL-10）、四种C-C趋化因子（MIP-1α、MIP-1β、MCAF和RANTES）以及两种C-X-C趋化因子（IL-8和GRO-α）的mRNA表达显著降低。