Roselli C E, Klosterman S, Resko J A
Department of Physiology & Pharmacology, Oregon Health Science University, Portland, Oregon 97201-3098, USA.
J Comp Neurol. 2001 Oct 15;439(2):208-23. doi: 10.1002/cne.1343.
This study mapped the regional locations of cells expressing cytochrome P450 aromatase (P450AROM) and androgen receptor (AR) mRNAs in the adult male macaque hypothalamus and amygdala by in situ hybridization histochemistry using monkey-specific cRNA probes. High densities of P450AROM and AR mRNA-containing neurons were observed in discrete hypothalamic areas involved in the regulation of gonadotropin secretion and reproductive behavior. P450AROM mRNA-containing neurons were most abundant in the medial preoptic nucleus, bed nucleus of the stria terminalis, and anterior hypothalamic area, whereas AR mRNA-containing neurons were most numerous in the ventromedial nucleus, arcuate nucleus, and tuberomamillary nucleus. Moderate to heavily labeled P450AROM mRNA-containing cells were present in the cortical and medial amygdaloid nuclei, which are known to have strong reciprocal inputs with the hypothalamus. Heavily labeled P450AROM mRNA-containing cells were found in the accessory basal amygdala nucleus, which projects to the cingulate cortex and hippocampus, areas that are important in the expression of emotional behaviors and memory processing. In contrast to P450AROM, the highest density of AR mRNA labeling in the temporal lobe was associated with the cortical amygdaloid nucleus and the pyramidal cells of the hippocampus. All areas that contained P450AROM mRNA-expressing cells also contained AR mRNA-expressing cells, but there were areas in which AR mRNA was expressed but not P450AROM mRNA. The apparent relative differences in the expression of P450AROM and AR mRNA-containing neurons within the monkey brain suggests that T acts through different signaling pathways in specific brain areas or within different cells from the same region.
本研究通过使用猴特异性cRNA探针的原位杂交组织化学方法，绘制了成年雄性猕猴下丘脑和杏仁核中表达细胞色素P450芳香化酶（P450AROM）和雄激素受体（AR）mRNA的细胞的区域定位。在参与促性腺激素分泌调节和生殖行为的离散下丘脑区域中，观察到高密度的含P450AROM和AR mRNA的神经元。含P450AROM mRNA的神经元在内侧视前核、终纹床核和下丘脑前区最为丰富，而含AR mRNA的神经元在腹内侧核、弓状核和乳头体核中数量最多。中度至重度标记的含P450AROM mRNA的细胞存在于皮质杏仁核和内侧杏仁核中，已知这些区域与下丘脑有强烈的相互输入。在副基底杏仁核中发现了重度标记的含P450AROM mRNA的细胞，该核投射到扣带回皮质和海马体，这些区域在情绪行为表达和记忆处理中很重要。与P450AROM不同，颞叶中AR mRNA标记的最高密度与皮质杏仁核和海马体的锥体细胞相关。所有含有表达P450AROM mRNA细胞的区域也都含有表达AR mRNA的细胞，但也有一些区域表达AR mRNA但不表达P450AROM mRNA。猴脑内P450AROM和含AR mRNA神经元表达的明显相对差异表明，睾酮在特定脑区或同一区域的不同细胞中通过不同的信号通路发挥作用。
