Roselli C E, Stormshak F, Resko J A
Department of Physiology and Pharmacology, School of Medicine, Oregon Health Sciences University, Portland, Oregon 97201-3098, USA.
J Neuroendocrinol. 2000 Jul;12(7):656-64. doi: 10.1046/j.1365-2826.2000.00496.x.
The regional distribution of neurones expressing aromatase mRNA in the ram hypothalamus was examined by in situ hybridization using 33P-labelled cRNA probes. The highest amounts of hybridization signal were observed in the central part of the medial preoptic nucleus and posterior medial part of the bed nucleus of the stria terminalis. Moderate amounts of hybridization signal were observed in the anteroventral periventricular preoptic nucleus, medial preoptic nucleus and a broad band extending between the medial preoptic nucleus and bed nucleus of the stria terminalis. Low levels of hybridization signal were observed in the organum vasculosum of the lamina terminalis, anterior part of the medial preoptic nucleus, and central part of the ventromedial nucleus of the hypothalamus. The presence of aromatase mRNA within neurones of the steroid-sensitive hypothalamic circuit supports a role for aromatization in the mechanism of testosterone action on reproductive function in male sheep. The distribution of aromatase mRNA in the ovine hypothalamus was similar to that described for other vertebrate species, suggesting a high degree of functional conservation across species.
使用33P标记的cRNA探针，通过原位杂交技术研究了在公羊下丘脑表达芳香化酶mRNA的神经元的区域分布。在内侧视前核的中央部分和终纹床核的后内侧部分观察到最高量的杂交信号。在前腹侧室周视前核、内侧视前核以及在内侧视前核和终纹床核之间延伸的一条宽带中观察到中等量的杂交信号。在终板血管器、内侧视前核的前部和下丘脑腹内侧核的中央部分观察到低水平的杂交信号。类固醇敏感的下丘脑回路神经元内芳香化酶mRNA的存在支持了芳香化作用在睾酮对雄性绵羊生殖功能作用机制中的作用。芳香化酶mRNA在绵羊下丘脑中的分布与其他脊椎动物物种所描述的相似，表明跨物种具有高度的功能保守性。
