高效液相色谱法测定人尿中右美沙芬及其代谢物
High performance liquid chromatographic determination of dextromethorphan and its metabolite in human urine.
作者信息
Cai W M, Chen B, Chu X
机构信息
Department of Clinical Pharmacology, Nanjing General Hospital of Nanjing Command PLA, Nanjing 210002.
出版信息
Yao Xue Xue Bao. 1997 Nov;32(11):861-4.
A reverse-phase high-performance liquid chromatographic method was established for the determination of dextromethorphan (DM) and its metabolite dextrophan (DX) in human urine. The sample was purified by an acid-base extraction followed by HPLC analysis on a phenyl column. The eluate was monitored by fluorescence at 280 nm (excitation) and 310 nm (emission). The limit of detection of DM was 0.023 mg.L-1. The within-day and between-day RSD% of DM and DX were all lower than 5%. The average recovery was 104.78%.
建立了一种反相高效液相色谱法，用于测定人尿中右美沙芬（DM）及其代谢产物右啡烷（DX）。样品经酸碱萃取纯化后，在苯基柱上进行高效液相色谱分析。洗脱液在280nm（激发）和310nm（发射）处用荧光监测。DM的检测限为0.023mg.L-1。DM和DX的日内和日间相对标准偏差均低于5%。平均回收率为104.78%。
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